Biolegend, 309824, Alexa Fluor® 647 anti-human CD137 (4-1BB) Antibody
100tests
Catalog Number: 309824
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| Verified Reactivity | Human |
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| Reported Reactivity | Chimpanzee, Baboon, Cynomolgus, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Ectodomain of recombinant human 4-1BB fusion protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, inhibition of cytokine production2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)). |
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| Application References(PubMed link indicates BioLegend citation) | Garni-Wagner B, et al. 1996. Cell. Immunol. 169:91. (IP) Salih HR, et al. 2000. J. Immunol. 165:2903. (FA) Kienzle G, et al. 2000. Int. Immunol. 12:73. (FA) Langstein J, et al. 1998. J. Immunol. 160:2488. (IP) |
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| Product Citations | Geuijen C, et al. 2021. Nat Commun. 12:4445. PubMed Goletz C, et al. 2018. Front Immunol. 9:1614. PubMed Charmetant X, et al. 2022. Sci Transl Med. :eabl6141. PubMed Meckiff BJ, et al. 2020. Cell. 183(5):1340-1353.e16. PubMed Nguyen J, et al. 2021. Mol Syst Biol. 17:e10560. PubMed Franchini DM et al. 2019. Cell reports. 26(1):94-107 . PubMed |
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| RRID | AB_2566257 (BioLegend Cat. No. 309823) AB_2566258 (BioLegend Cat. No. 309824) |
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| Structure | TNFR superfamily, type I transmembrane protein, 30 kD |
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| Distribution | Activated T cells |
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| Function | T cell costimulation |
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| Ligand/Receptor | 4-1BB ligand |
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| Cell Type | T cells |
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| Biology Area | Costimulatory Molecules, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Gruss H, et al. 1995. Blood 85:3378. 2. Sica G, et al. 2000. Adv. Exp. Med. Biol. 465:355. 3. Alderson M, et al. 1994. Eur. J. Immunol. 24:2219. 4. Schwarz H, et al. 1996. Blood 87:2839. |
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| Gene ID | 3604 |
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| UniProt | View information about CD137 on UniProt.org |
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| Clone | 4B4-1 |
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| Regulatory Status | RUO |
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| Workshop | VI C-7 |
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| Other Names | 4-1BB, ILA, CD137, TNFRSF9 |
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| Isotype | Mouse IgG1, κ |
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