Biolegend, 310709, Alexa Fluor® 647 anti-human CD193 (CCR3) Antibody
25tests
Catalog Number: 310709
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: The 5E8 antibody is useful for immunofluorescent staining and flow cytometric analysis of CCR3 expression. It has been observed that the 5E8 antibody clone can interact with PE/Cyanine7 antibody conjugates during multi-color staining, potentially leading to unwanted staining. This interaction can be resolved by sequentially staining with the 5E8 antibody first and then followed by the PE/Cyanine7 conjugate of interest. |
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| Application References(PubMed link indicates BioLegend citation) | Beauvillian C, et al. 2011. Blood 117:1196. PubMed |
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| Product Citations | Bastian OW, et al. 2018. Front Immunol. 9:945. PubMed Lee P, et al. 2011. J Immunol. 186:1747. PubMed Rohner L, et al. 2020. Sci Rep. 0.570833333. PubMed Dunn JLM, et al. 2021. Mucosal Immunol. 14:1133. PubMed Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed Engelhard A 2010. J Immunol. 184:4079. PubMed |
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| RRID | AB_2275699 (BioLegend Cat. No. 310709) AB_2074380 (BioLegend Cat. No. 310710) |
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| Structure | G-protein coupled seven transmembrane domain receptor, 356 amino acids |
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| Distribution | Eosinophils, basophils, mast, mononuclear phagocytes, platelets, CD34+ hematopoietic progenitor, Th2, keratinocytes |
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| Function | Co-receptor for HIV-1 and HIV-2, allergy |
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| Receptors | Eotaxin, eotaxin-2, eotaxin-3 |
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| Cell Type | Eosinophils, Erythrocytes, Hematopoietic stem and progenitors, Macrophages, Mast cells, Thymocytes |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors, GPCR |
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| Antigen References | 1. Gerard W, et al. 1996. J. Exp. Med. 183:2437. 2. Uguccioni C, et al. 1997. J. Clin. Invest. 100:1137. 3. Sallusto F, et al. 1997. Science. 277:2005. 4. Loetscher P, et al. 2001. J. Biol. Chem. 276:2986. |
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| Regulation | Upregulated by high affinity Fc IgE receptor ligation (mast cells), RANTES (keratinocytes), IFNg (monocytes, neutrophils), HIV tat protein (basophils), IL-3, IL-5 and GM-CSF (CD34+ progenitor cells), IL-2 and IL-4(T cells). Downregulated by IL- |
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| Gene ID | 1232 |
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| UniProt | View information about CD193 on UniProt.org |
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| Clone | 5E8 |
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| Regulatory Status | RUO |
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| Other Names | CC CKR3, MIP1-alpha receptor like-2, eotaxin receptor, CD193, CCR3 |
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| Isotype | Mouse IgG2b, κ |
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