Biolegend, 310818, Alexa Fluor® 647 anti-human CD154 (CD40L) Antibody
100tests
Catalog Number: 310818
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Category
Pack Size40 L
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| Verified Reactivity | Human |
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| Reported Reactivity | Cynomolgus, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1,3 and blocking of T cell-dependent B cell differentiation1,2,4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 310812). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 310828) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg). |
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| Application References(PubMed link indicates BioLegend citation) | Brams P, et al. 2001. Int. Immunopharmacol. 1:277. (Block, IF) Rushworth SA, et al. 2002. Transplantation 73:635. (Block) Berner B, et al. 2000. Ann. Rheum. Dis. 59:190. (IF) Nordström T, et al. 2006. J. Leukocyte Biol. 79:319. (Block) Zhang AL, et al.2007. Blood doi:10.1182/blood-2007-02-076364. (Block) PubMed Kuchen S, et al. 2007. J. Immunol. 179:5886. Matus-Nicodermos R, et al. 2011. J. Immunol. 186:2164. PubMed Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG) |
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| Product Citations | Paniskaki K, et al. 2022. Transplant Proc. 54:1455. PubMed Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed Thieme CJ, et al. 2020. Cell Rep Med. 1:100092. PubMed Céspedes PF, et al. 2022. Nat Commun. 13:3460. PubMed |
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| RRID | AB_492970 (BioLegend Cat. No. 310818) |
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| Structure | TNF superfamily, type II transmembrane glycoprotein, cleaved as soluble CD40L, 32-39 kD |
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| Distribution | Activated T cells |
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| Function | B cell costimulation |
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| Ligand/Receptor | CD40 |
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| Cell Type | T cells, Tregs |
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| Biology Area | Costimulatory Molecules, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Najafian N, et al. 2003. Expert Opin. Biol. Ther. 3:227. 2. Racke M, et al. 2002. Expert Opin. Ther. Targets. 6:275. 3. Ford G, et al. 1999. J. Immunol. 162:4037. |
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| Gene ID | 959 |
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| UniProt | View information about CD154 on UniProt.org |
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| Clone | 24-31 |
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| Regulatory Status | RUO |
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| Other Names | CD40L, gp39, TRAP, T-BAM, TNFSF5 |
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| Isotype | Mouse IgG1, κ |
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