Biolegend, 311414, Alexa Fluor® 647 anti-human HLA-A,B,C Antibody
100tests
Catalog Number: 311414
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| Verified Reactivity | Human, Cynomolgus, Rhesus |
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| Reported Reactivity | African Green, Baboon, Cat, Cow, Chimpanzee |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule21.Additional reported applications (for the relevant formats) include: immunoprecipitaton2, Western blotting (non-reducing)3, immunohistochemical staining of acetone-fixed frozen tissue sections4,5, blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg). |
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| Application References(PubMed link indicates BioLegend citation) | Darrow TL, et al. 1989. J. Immunol. 142:3329. Stern P, et al. 1987. J. Immunol. 138:1088. Tran TM, et al. 2001. Immunogenetics 53:440. Barbatis C, et al. 1981. Gut 22:985. Ayyoub M, et al. 2004. Cancer Immunity 4:7. DeFelice M, et al. 1990. Cell. Immunol. 126:420. Fayen J, et al. 1998. Int. Immunol. 10:1347. Turco MC, et al. 1988. J. Immunol. 141:2275. Geppert TD, et al. 1989. J. Immunol. 142:3763. Wooden SL, et al. 2005. J. Immunol. 175:1383. Nagano M, et al. 2007. Blood 110:151. McLoughlin RM,et al.2008. J. Immunol. 181:1323. PubMed Takahara M, et al.2008. J. Leukoc. Biol. 83:742. PubMed Lunemann A, et al.2008. J. Immunol. 181:6170. PubMed Laing BJ, et al. 2010. J. Thorac Cardiovasc Surg. 139:1402. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Vambutas A, et al. 2000. Clin. Diagn. Lab. Immun. 7:79. Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (epitope) Crivello P, et al. 2013. Hum Immunol. 22:100. PubMed Jung Y, et al. 2015. Mol Cancer Res. 13:197. PubMed Shields MJ. Ribaudo RK. 1998. Tissue Antigens. 51(5):567-70. (epitope) |
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| Product Citations | Porat-Shliom N, et al. 2013. PLoS One. 25:81897. PubMed Sparbier CE, et al. 2023. Nat Cell Biol. 25:258. PubMed Gamage TKJB, et al. 2018. Biol Open. 7. PubMed Cao J, et al. 2018. Invest Ophthalmol Vis Sci. 59:1005. PubMed Ma A, et al. 2019. Hum Vaccin Immunother. :1. PubMed Souri Z, et al. 2020. Cancers (Basel). 12: . PubMed Thomas L, et al. 2016. Mol Cancer Ther. 15(12):2946-2954. PubMed Siciliano V, et al. 2018. Nat Commun. 9:1881. PubMed Reichel J, et al. 2015. Blood. 125:1061. PubMed James J, et al. 2015. Reproduction. 150: 449 - 462. PubMed Stewart CA, et al. 2020. Nat Cancer. 423:1. PubMed Bonfá G, et al. 2021. Methods Mol Biol. 2229:331. PubMed Cornel AM, et al. 2020. Cytometry A. 97:845. PubMed Bernson E, et al. 2019. Front Immunol. 2.113888889. PubMed Gay CM, et al. 2021. Cancer Cell. 39:346. PubMed |
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| RRID | AB_493136 (BioLegend Cat. No. 311416) AB_493135 (BioLegend Cat. No. 311414) |
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| Structure | Ig superfamily |
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| Distribution | All nucleated cells |
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| Function | Antigen presentation |
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| Ligand/Receptor | CD3/TCR, CD8 |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | MHC Antigens |
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| Antigen References | 1. Barclay AN, et al. Eds. 1993. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego. |
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| Gene ID | 3105 |
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| UniProt | View information about HLA-A on UniProt.org |
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| Clone | W6/32 |
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| Regulatory Status | RUO |
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| Other Names | Major Histocompatibility Class I, MHC class I |
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| Isotype | Mouse IgG2a, κ |
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