Biolegend, 313234, Alexa Fluor® 488 anti-human CD117 (c-kit) Antibody
100tests
Catalog Number: 313234
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| Verified Reactivity | Human |
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| Reported Reactivity | Cynomolgus, Cow |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | MOLM-1 megakaryocytic cell line |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested SB - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy1, and spatial biology (IBEX)4,5. |
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| Additional Product Notes | Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH). |
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| Application References(PubMed link indicates BioLegend citation) | Broudy VC, et al. 1999. Blood 94:1979. (IF, IP) Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Nagano M, et al. 2007. Blood 110:151. (FC) PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Wegmann R, et al. 2024. Nat Commun. 9402:15. PubMed |
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| RRID | AB_2566220 (BioLegend Cat. No. 313233) AB_2566221 (BioLegend Cat. No. 313234) |
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| Structure | Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD |
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| Distribution | Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML) |
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| Function | Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells. |
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| Ligand/Receptor | c-Kit ligand |
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| Modification | Multiple phosphorylation sites |
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| Cell Type | Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells |
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| Biology Area | Immunology, Stem Cells |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Giebel LB, et al. 1992. Oncogene 7:2207. 2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736. |
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| Gene ID | 3815 |
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| UniProt | View information about CD117 on UniProt.org |
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| Clone | 104D2 |
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| Regulatory Status | RUO |
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| Other Names | Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor |
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| Isotype | Mouse IgG1, κ |
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