Biolegend, 313245, Alexa Fluor® 700 anti-human CD117 (c-kit) Antibody
25tests
Catalog Number: 313245
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| Verified Reactivity | Human |
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| Reported Reactivity | Cynomolgus, Cow |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | MOLM-1 megakaryocytic cell line |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy1, and spatial biology (IBEX)4,5. |
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| Application References(PubMed link indicates BioLegend citation) | Broudy VC, et al. 1999. Blood 94:1979. (IF, IP) Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Nagano M, et al. 2007. Blood 110:151. (FC) PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| RRID | AB_2860833 (BioLegend Cat. No. 313245) AB_2860834 (BioLegend Cat. No. 313246) |
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| Structure | Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD |
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| Distribution | Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML) |
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| Function | Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells. |
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| Ligand/Receptor | c-Kit ligand |
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| Modification | Multiple phosphorylation sites |
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| Cell Type | Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells |
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| Biology Area | Immunology, Stem Cells |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Giebel LB, et al. 1992. Oncogene 7:2207. 2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736. |
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| Gene ID | 3815 |
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| UniProt | View information about CD117 on UniProt.org |
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| Clone | 104D2 |
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| Regulatory Status | RUO |
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| Other Names | Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor |
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| Isotype | Mouse IgG1, κ |
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