Biolegend, 313331, Alexa Fluor® 647 anti-human CD135 (Flt-3/Flk-2) Antibody
25tests
Catalog Number: 313331
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Category
| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | BV-173 pro-B cell line |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| RRID | AB_3683326 (BioLegend Cat. No. 313331) AB_3683326 (BioLegend Cat. No. 313332) |
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| Structure | Member of the immunoglobulin supergene family, type 3 tyrosine kinase receptor; 130-160 kD |
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| Distribution | Expressed on CD34+ hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. Also expressed on AML, ALL and CML-BC tumor cells |
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| Function | Tyrosine kinase growth factor receptor involved in the growth and differentiation of primitive hematopoietic cells |
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| Ligand/Receptor | FLT3 ligand |
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| Cell Type | B cells, Hematopoietic stem and progenitors, Leukemia, Thymocytes |
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| Biology Area | Cell Biology, Immunology |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors |
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| Antigen References | 1. Rappold I, et al. 1997. Blood 90:111. 2. Rosnet O, et al. 1996. Acta Haematol. 95:218. 3. Rosnet O, et al. 1996. Leukemia 10:238. 4. Bertho JM, et al. 2000. Scand. J. Immunol. 52:53. |
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| Gene ID | 2322 |
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| UniProt | View information about CD135 on UniProt.org |
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| Clone | BV10A4H2 |
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| Regulatory Status | RUO |
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| Other Names | FMS-like tyrosine kinase-3, FLT-3, STK-1, Flk-2 |
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| Isotype | Mouse IgG1, κ |
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