Biolegend, 313514, Alexa Fluor® 488 anti-human/mouse/rat CD278 (ICOS) Antibody
100microg
Catalog Number: 313514
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| Verified Reactivity | Human, Mouse, Rat |
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| Reported Reactivity | African Green, Baboon, Cynomolgus, Rhesus, Pig |
| Antibody Type | Monoclonal |
| Host Species | Armenian Hamster |
| Immunogen | Mouse T cell clone D10.G4.1 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested SB - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that reagents be titrated for optimal performance in the particular application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 313512). |
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| Additional Product Notes | Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH). |
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| Application References(PubMed link indicates BioLegend citation) | Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. (FC IP Costim) Yagi J, et al. 2003. J. Immunol. 171:783. (FC) Arimura Y, et al. 2002. Int. Immunol. 14:555. (Costim) Arimura Y, et al. 2004. J. Biol. Chem. 279:11408. (Costim) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Matsui K, et al. 2015. PLoS One. 10: 0137195. PubMed Yue X, et al. 2019. Nat Commun. 10:2011. PubMed Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed Masuda N, et al. 2021. BMC Cancer. 21:1269. PubMed Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed |
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| RRID | AB_2122584 (BioLegend Cat. No. 313514) |
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| Structure | CD28/CTLA-4, 47-57 kD |
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| Distribution | Activated T cells, subset of thymocytes |
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| Function | Costimulates T cell activation, proliferation, humoral immune response |
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| Ligand/Receptor | B7h/B7RP-1/GL-50 |
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| Cell Type | T cells, Thymocytes, Tregs |
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| Biology Area | Costimulatory Molecules, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. 2. Hutloff A, et al. 1999. Nature 397:263. 3. Buonfiglio D, et al. 2000. Eur. J. Immunol. 30:3463. 4. Coyle AJ, et al. 2000. Immunity 13:95. |
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| Gene ID | 1000488412985164545 |
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| UniProt | View information about CD278 on UniProt.org |
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| Clone | C398.4A |
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| Regulatory Status | RUO |
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| Other Names | Inducible COStimulatory molecule, H4 |
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| Isotype | Armenian Hamster IgG |
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