Biolegend, 314535, Alexa Fluor® 647 anti-human IgM Antibody
25tests
Catalog Number: 314535
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| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Baboon, Cynomolgus, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human Ig cocktail |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis. Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended. |
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| Application References(PubMed link indicates BioLegend citation) | Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed |
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| Product Citations | Bradley T et al. 2018. Cell. 175(2):387-399 . PubMed Iype J, et al. 2019. J Immunol. 202:1417. PubMed |
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| RRID | AB_2566612 (BioLegend Cat. No. 314535) AB_2566613 (BioLegend Cat. No. 314536) |
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| Structure | Ig family |
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| Distribution | B cells |
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| Function | B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis |
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| Cell Type | B cells |
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| Biology Area | Immunology |
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| Gene ID | 3507 |
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| UniProt | View information about IgM on UniProt.org |
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| Clone | MHM-88 |
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| Regulatory Status | RUO |
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| Other Names | Immunoglobulin M |
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| Isotype | Mouse IgG1, κ |
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