Biolegend, 317357, Cell-Vive™ GMP Ultra-LEAF™ Biotin anti-human CD3 SF Antibody
100microg
Catalog Number: 317357
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| Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | 0.1 μm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test. |
| Endotoxin Level | Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test |
| Preparation | The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography. |
| Concentration | 1.0 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes |
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| GMP Ultra-LEAF™ Biotin anti-human CD3 SF Antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.1 μm filtered) is recommended for highly sensitive assays. The antibody was GMP manufactured under serum-free conditions including serum-free hybridoma cell culture, without additional animal or human-derived materials or preservatives. This antibody is intended for flow cytometry and for research or further ex vivo bioprocessing use only. The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. Clone OKT3 can block the binding of clones SK7 and UCHT14. The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. |
| Additional Product Notes | For plate coating protocols, Biotin anti-human CD3 antibody (Cat. No. 317357/317358) can be used at 1 μg/mL in PBS in combination with 1 μg/mL of Biotin anti-human CD28 antibody (Cat. No. 302978) to efficiently activate T cells. Plates should be coated for 2 hours in a tissue culture incubator or overnight at 4°C. For soluble antibody activation protocols, we recommend 10 μg/mL of anti-human CD3 (Cat. No. 317357/317358) antibody and 5 μg/mL of anti-human CD28 antibody (Cat. No. 302978). |
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| Disclaimer | BioLegend Cell-Vive™ GMP Ultra-LEAF™ antibodies are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. |
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| Structure | Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) form the CD3/TCR complex, 20 k |
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| Distribution | Mature T and NK T cells, thymocyte differentiation |
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| Function | Antigen recognition, signal transduction, T cell activation |
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| Ligand/Receptor | Peptide antigen bound to MHC |
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| Cell Type | NKT cells, T cells, Thymocytes, Tregs |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. Lanier L, et al. 1986. J. Immunol. 137:2501. |
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| Gene ID | 916 |
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| UniProt | View information about CD3 on UniProt.org |
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| Clone | OKT3 |
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| Other Names | T3, CD3ε |
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| Isotype | Mouse IgG2a, κ |
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