Biolegend, 317420, Alexa Fluor® 488 anti-human CD4 Antibody
100tests
Catalog Number: 317420
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| Verified Reactivity | Human, Cynomolgus, Rhesus |
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| Reported Reactivity | Chimpanzee |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human peripheral T cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality testedIHC-F - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454). In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9 |
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| Application References(PubMed link indicates BioLegend citation) | Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36. Linder J, et al. 1987. Am. J. Pathol. 127:1. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC) Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen) Lederman S, et al. 1991. Mol Immunol. 28:1171-81. |
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| Product Citations | Sugrue JA, et al. 2022. Cell Rep Med. 3:100804. PubMed Mascarau R, et al. 2023. J Cell Biol. 222:. PubMed Ellis GI, et al. 2022. Cell Rep Med. 3:100614. PubMed Demirkhanyan L, et al. 2012. J Biol Chem. 287:28821. PubMed Johnson RF, et al. 2021. Viruses. 13:. PubMed Rademacher MJ, et al. 2021. Sci Rep. 11:8321. PubMed Porsche CE, et al. 2021. JCI Insight. 6:. PubMed Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed Abe S, et al. 2020. JCI Insight. 5:00. PubMed Kraig E, et al. 2018. Exp Gerontol. 105:53. PubMed Zhou H, et al. 2022. EMBO Rep. 23:e53475. PubMed Meyer LK, et al. 2019. J Clin Invest. 130:863. PubMed Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed Billing F, et al. 2021. ACS Appl Mater Interfaces. 13:55534. PubMed Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed Maehara T, et al. 2018. Life Sci Alliance. 1:e201800050. PubMed Singh A, et al. 2020. Cell Rep. 32:108153. PubMed Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed Sharei A, et al. 2015. PLoS One. 10:118803. PubMed |
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| RRID | AB_571938 (BioLegend Cat. No. 317419) AB_571939 (BioLegend Cat. No. 317420) |
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| Structure | Ig superfamily, type I transmembrane glycoprotein, 55 kD |
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| Distribution | T cell subset, majority of thymocytes, monocytes/macrophages |
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| Function | MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor |
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| Ligand/Receptor | MHC class II molecules, HIV gp120, IL-16 |
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| Cell Type | Macrophages, Monocytes, T cells, Thymocytes, Tregs |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Center D, et al. 1996. Immunol. Today 17:476. 2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723. |
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| Gene ID | 920 |
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| UniProt | View information about CD4 on UniProt.org |
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| Clone | OKT4 |
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| Regulatory Status | RUO |
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| Workshop | HCDM listed |
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| Other Names | T4 |
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| Isotype | Mouse IgG2b, κ |
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