Biolegend, 317422, Alexa Fluor® 647 anti-human CD4 Antibody
100tests
Catalog Number: 317422
Send an RFQ.
Category
Loading…
| Verified Reactivity | Human, Cynomolgus, Rhesus |
|---|---|
| Reported Reactivity | Chimpanzee |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human peripheral T cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested IHC-F - Verified |
| Recommended Usage |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Red Laser (633 nm) |
|---|
| Application Notes | The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454). In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9 |
|---|
| Application References(PubMed link indicates BioLegend citation) | Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36. Linder J, et al. 1987. Am. J. Pathol. 127:1. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC) Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen) Lederman S, et al. 1991. Mol Immunol. 28:1171-81. |
|---|
| Product Citations | Izquierdo-Useros N, et al. 2012. PLoS Biol. 10:e1001448. PubMed Greenwood EJD et al. 2019. Cell Rep. 27(5):1579-1596 . PubMed Baleeiro RB, et al. 2022. Oncoimmunology. 11:2080329. PubMed Veazey R, et al. 2016. Mucosal Immunol. 10.1038/mi.2015. PubMed Carter JA, et al. 2020. Cell Systems. 9(5):475-482.e4.. PubMed |
|---|
| RRID | AB_571941 (BioLegend Cat. No. 317422) |
|---|
| Structure | Ig superfamily, type I transmembrane glycoprotein, 55 kD |
|---|
| Distribution | T cell subset, majority of thymocytes, monocytes/macrophages |
|---|
| Function | MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor |
|---|
| Ligand/Receptor | MHC class II molecules, HIV gp120, IL-16 |
|---|
| Cell Type | Macrophages, Monocytes, T cells, Thymocytes, Tregs |
|---|
| Biology Area | Immunology |
|---|
| Molecular Family | CD Molecules |
|---|
| Antigen References | 1. Center D, et al. 1996. Immunol. Today 17:476. 2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723. |
|---|
| Gene ID | 920 |
|---|
| UniProt | View information about CD4 on UniProt.org |
|---|
| Clone | OKT4 |
|---|
| Regulatory Status | RUO |
|---|
| Workshop | HCDM listed |
|---|
| Other Names | T4 |
|---|
| Isotype | Mouse IgG2b, κ |
|---|
