Biolegend, 318314, Alexa Fluor® 647 anti-human CD56 (NCAM) Antibody
100tests
Catalog Number: 318314
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| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Baboon, Cynomolgus, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Clone HCD56 is not recommended for immunohistochemistry formalin-fixed paraffin-embedded tissue. |
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| Application References(PubMed link indicates BioLegend citation) | Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed |
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| Product Citations | Gunesch JT, et al. 2020. Elife. 9: . PubMed Crespo ÂC, et al. 2020. Cell. 182(5):1125-1139. PubMed Kuang YL, et al. 2019. Stem Cell Res. 37:101434. PubMed Mace E, et al. 2016. Nat Commun. 7:12171. PubMed Perros F, et al. 2013. Am J Respir Crit Care Med. 187:189. PubMed Pham TNQ, et al. 2020. Cell Reports. 29(9):2770-2782.e5.. PubMed |
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| RRID | AB_604095 (BioLegend Cat. No. 318313) AB_604103 (BioLegend Cat. No. 318314) |
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| Structure | Ig superfamily, single transmembrane or GPI-anchored glycoprotein |
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| Distribution | NK cells, T subset, neural tissue, some LGL and myeloid leukemias |
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| Function | Adhesion |
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| Ligand/Receptor | Heparin sulfate |
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| Cell Type | B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells |
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| Biology Area | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Lanier L, et al. 1991. J. Immunol. 146:4421. 2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71. 3. Cremer H, et al. 1994. Nature 367:455. |
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| Gene ID | 4684 |
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| UniProt | View information about CD56 on UniProt.org |
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| Clone | HCD56 |
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| Regulatory Status | RUO |
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| Other Names | Leu-19, NKH1 |
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| Isotype | Mouse IgG1, κ |
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