Biolegend, 320027, True-Nuclear™ Human Treg Flow Kit
25tests
Catalog Number: 320027
Send an RFQ.
Category
Loading…
| Verified Reactivity | Human |
|---|---|
| Antibody Type | Monoclonal |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | This kit is guaranteed for six months. Upon receipt, store between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Application Notes |
|---|
| Materials Provided: 1. Alexa Fluor® 488 anti-mouse/rat/human FOXP3 - 25 tests 2. Alexa Fluor® 488 Mouse IgG1, κ isotype control - 25 tests 3. True-Nuclear™ Transcription Factor Buffer Set - 120 tests (Cat. No. 424401) 4. anti-human CD4 PE-Cyn5/CD25 PE Cocktail - 50 tests Materials Not Included: 1. Cell Staining Buffer (Cat. No. 420201) 2. Single color compensation controls Immunofluorescence Staining Procedures: 1. Perform cell surface staining as described in BioLegend's Cell Surface Immunofluorescence Staining Protocol. Add 20 µL of the anti-human CD4 PE-Cyn5/CD25 PE cocktail to each tube and incubate in the dark for 20 minutes. 2. Add 2 mL of the cell staining buffer, centrifuge tubes at 400 x g at room temperature for five minutes, and discard the supernatant. 3. Repeat Step 2, for a total of two washes. 4. Add 1 mL of the Transcription Factor 1X Fix solution to each tube, vortex, and incubate at room temperature in the dark for 45-60 minutes. 5. Without washing, add 2 mL of the Transcription Factor 1X Perm Buffer to each tube. 6. Centrifuge tubes at 400 x g at room temperature for five minutes, and discard the supernatant. 7. Add 2 mL of the Transcription Factor 1X Perm Buffer to each tube. 8. Centrifuge tubes at 400 x g at room temperature for five minutes, and discard the supernatant. 9. Resuspend the cell pellet in 100 µL of the Transcription Factor 1XPerm Buffer. 10. Add 5 µL of Alexa Fluor® 488 anti-mouse/rat/human FOXP3 antibody or 5 µL of Alexa Fluor® 488 mouse IgG1, κ isotype control into the appropriate tubes. Incubate in the dark at room temperature for at least 30 minutes. 11. Add 2 mL of the Transcription Factor 1X Perm Buffer to each tube. 12. Centrifuge tubes at 400 x g at room temperature for five minutes, and discard the supernatant. 13. Add 2 mL of the cell staining buffer. 14. Centrifuge tubes at 400 x g at room temperature for five minutes, and discard the supernatant. 15. Resuspend in 0.5 mL cell staining buffer and then acquire tubes on a flow cytometer. Caution: The True-Nuclear™ Transcription Factor Buffer Set contains paraformaldehyde, which is toxic and mutagenic. Please handle with caution. Wear gloves, lab coats, and necessary protection to avoid direct contact. NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3/Perm Buffer Set (Cat. No. 421403). |
| Application References(PubMed link indicates BioLegend citation) | Roncador G, et al. 2005 Eur. J. Immunol. 35:1681. Mayack. S,et al. 2006. J. Immunol.176:2059. J. Immunol Yang ZZ, et al. 2006. Blood 107:3639. Gavin MA, et al. 2006. P. Natl. Acad. Sci. USA 103:6659. Groh V, et al. 2006. Nature Immunology 7:755. Lewkowicz P, et al. 2006 J. Immunol. 177:7155. Luke PPW, et al. 2006. Amer. J. Transplant. 6(9):2023. Bamias G, et al. 2007. J. Immunol. 178:1809. Valencia X, et al. 2007. J. Immunol. 178:2579.PubMed Davidson TS, et al. 2007. J. Immunol. 178:4022. MacDonald K PA, et al. 2007. Blood doi:10.1182/blood-2007-01-067249. Jaffar Z, et al. 2007. J. Immunol. 179:6193. Müller M, et al. 2007. J. Immunol. 179:2774. Jordan JM,et al. 2008.Infect Human. 76:3717. PubMed Golovina TN,et al. 2008. J. Immunol. 181:2855. PubMed Fallarino F, et al. 2009. J. Exp Med. 206:2511. PubMed Banham Alison, et al. 2009. Vet Immunol and Immunop 127.3-4:376-381 Klunker S, et al. 2009. J. Exp Med. PubMed Haque A, et al. 2010. J. Immunol. 184:2583. PubMed Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed " |
|---|
| RRID | AB_3097007 (BioLegend Cat. No. 320027) |
|---|
| Distribution | T regulatory cells |
|---|
| Cell Type | Tregs |
|---|
| Biology Area | Cell Biology, Immunology, Transcription Factors |
|---|
| Molecular Family | CD Molecules, Nuclear Markers |
|---|
| Antigen References | 1. Hori S, et al. 2003. Science 299:1057. 2. Fontenot JD, et al. 2003. Nature Immunol. 4:330. 3. Ferguson PJ, et al. 2000. Am. J. Med. Genet. 90:390. 4. Bennett CL, et al. 2001. Nature Genet. 27:20. 5. Allan SE, et al. 2005. J. Clin. Invest. 115:3276. |
|---|
| Gene ID | 2037131738250943 |
|---|
| UniProt | View information about FOXP3 on UniProt.org |
|---|
| Clone | 150D |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2 |
|---|
