Biolegend, 320303, XerosFlow™ Human IP Backbone Kit
5tests
Catalog Number: 320303
Send an RFQ.
Category
Loading…
| Verified Reactivity | Human |
|---|---|
| Formulation | Each kit contains the following: 5 Tubes of Dry Human IP Backbone Cocktail (1 test/tube) 1 Compensation Control Tube for Spark Violet™ 538 (1 test/tube) 1 Compensation Control Tube for Alexa Fluor® 700 (1 test/tube) 1 Compensation Control Tube for PerCP/Cyanine5.5 (1 test/tube) 1 Compensation Control Tube for APC/Fire™ 750 (1 test/tube) 1 Compensation Control Tube for PE/Cyanine7 (1 test/tube) 1 Compensation Control Tube for PE/Dazzle™ 594 (1 test/tube) 1 Compensation Control Tube for FITC (1 test/tube) Dry cocktail is prepared from PBS containing stabilizers. Each tube can be reconstituted with 100 µL of whole blood/cell suspension or with 50 µL Cell Staining Buffer plus 50 µL of cell suspension. |
| Preparation | This kit is a combination of conjugated antibodies at optimal concentrations for flow cytometry application. |
| Storage & Handling | The product can be stored at ambient temperature upon receipt. Do not open the pouch until ready to use. Once opened, product can be stored at ambient temperature for up to a month in the mylar pouch. If product is reconstituted with buffer, it can be stored in the fridge (2 - 8°C) until it is ready to use (up to 1 week). |
| Application | FC - Quality tested |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Recommended Usage |
|---|
| Please refer to Application Notes |
| Application Notes | XerosFlow™ Human IP (Immunophenotyping) Backbone Kit is a dried down cocktail kit to characterize immune cell subsets. Components: 1 pouch containing 5 tests of XerosFlow™ Human IP Backbone Cocktail (1 test/tube) 1 set of Compensation Control tubes with lot-matched antibodies Required Materials Not Included: Cell Stain Buffer for Wash Steps (Cat. No. 420201 or similar) 1x RBC Lysis Buffer diluted from 10x RBC Lysis Buffer (Cat. No. 420302 or similar) 1% diluted Fixation Buffer from 4% if needed (Cat. No. 420801 or similar) Assay Protocol: Open the XerosFlow™ Human IP Backbone Kit pouches and the respective compensation control pouches and set up the dried down cocktail tubes to start the experiment Add 100 µL of human peripheral blood or similar cell suspension of interest into each of the dried down cocktail tubes and respective compensation controls and vortex thoroughly Incubate the samples at room temperature for 15 minutes If the cell suspension used does not need lysing, proceed to step 7. If performing Lyse wash whole blood assay, add 2 mL of 1x of RBC Lysis Buffer (Cat. No. 420302 or similar) to each tube and vortex thoroughly Incubate the samples at room temperature for 15 minutes Centrifuge the samples at 500 xg for 5 minutes, decant and vortex to loosen the pellet Add 2 mL Cell Stain Buffer (Cat. No. 420201 or similar) and centrifuge the samples at 500 xg for 5 minutes, decant and vortex to loosen the pellet Repeat step 7 Add 300 – 500 µL Cell Stain Buffer or 1% diluted Fixation Buffer (Cat. No. 420801 or similar) and acquire the samples using a flow cytometer |
|---|
| RRID | AB_3097207 (BioLegend Cat. No. 320303) |
|---|
| Distribution | T Cells, B cells, Granulocytes, Monocytes, NK Cells & Dendritic Cells |
|---|
| Molecular Family | CD Molecules |
|---|
| Gene ID | NA |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | Dry Reagents, Dry Cocktails |
|---|
