Biolegend, 320305, XerosFlow™ Human AIM Assay Cocktail Kit
5tests
Catalog Number: 320305
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| Verified Reactivity | Human |
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| Formulation | Each kit contains the following: 5 Tubes of XerosFlow™ Human AIM Assay Cocktail (1 test/tube) 1 Compensation Control Tube for Spark Violet™ 538 (1 test/tube) 1 Compensation Control Tube for APC/Fire™ 750 (1 test/tube) 1 Compensation Control Tube for Pacific Blue™ (1 test/tube) 1 Compensation Control Tube for FITC (1 test/tube) 1 Compensation Control Tube for Spark NIR™ 685 (1 test/tube) 1 Compensation Control Tube for PE/Dazzle™ 594 (1 test/tube) 1 Compensation Control Tube for PerCP/Cyanine5.5 (1 test/tube) 1 Compensation Control Tube for PE/Cyanine7 (1 test/tube) 1 Compensation Control Tube for PE (1 test/tube) Dry cocktail is prepared from PBS containing stabilizers. Each tube can be reconstituted with 100 µL of whole blood/cell suspension or with 50 µL Cell Staining Buffer plus 50 µL of cell suspension. |
| Preparation | This reagent is a combination of conjugated antibodies at optimal concentrations for flow cytometry application. |
| Storage & Handling | The product can be stored at ambient temperature upon receipt. Do not open the pouch until ready to use. Once opened, product can be stored at ambient temperature for up to a month in the mylar pouch. If product is reconstituted with buffer, it can be stored in the fridge (2 - 8°C) until it is ready to use (up to 1 week). |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Application | FC - Quality tested |
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| Recommended Usage | Please refer to Application Notes |
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| Application Notes | XerosFlow™ Human AIM (Activation Induced Markers) Assay Cocktail kit is a dried down cocktail kit that aims to characterize activated T cells subsets. Components: 1 pouch containing 5 tests of XerosFlow™ Human AIM Assay Cocktail (1 test/tube) 1 set of Compensation Control with lot-matched antibodies Required Materials Not Included: Cell Stain Buffer for Wash Steps (Cat. No. 420201 or similar) 1x RBC Lysis Buffer diluted from 10x RBC Lysis Buffer (Cat. No. 420302 or similar) 1% diluted Fixation Buffer from 4% if needed (Cat. No. 420801 or similar) Assay Protocol: Open the XerosFlow™ Human AIM Assay Cocktail Kit and the respective compensation control pouches and set up the dried down cocktail tubes to start the experiment Add 100 μL of human peripheral blood or similar cell suspension of interest into each of the dried down cocktail tubes and respective compensation controls and vortex thoroughly Incubate the samples at room temperature for 15 minutes If the cell suspension used does not need lysing, proceed to step 7. If performing Lyse wash whole blood, add 2 mL of 1x of RBC Lysis Buffer (Cat. No. 420302 or similar) to each tube and vortex thoroughly Incubate the samples at room temperature for 15 minutes Centrifuge the samples at 500 xg for 5 minutes, decant and vortex to loosen the pellet. Add 2 mL Cell Stain Buffer (Cat. No. 420201 or similar) and centrifuge the samples at 500 xg for 5 minutes, decant and vortex to loosen the pellet. Repeat step 7. Add 300 - 500 µL Cell Stain Buffer or 1% diluted Fixation Buffer (Cat. No. 420801 or similar) and acquire the samples using a flow cytometer. |
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| RRID | AB_3097203 (BioLegend Cat. No. 320305) |
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| Distribution | Activated T Cells |
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| Molecular Family | CD Molecules, Immune Checkpoint Receptors |
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| Gene ID | NA |
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| Regulatory Status | RUO |
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| Other Names | Dry Reagents, Dry Cocktails |
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