Biolegend, 320712, Alexa Fluor® 488 anti-human CD182 (CXCR2) Antibody
100tests
Catalog Number: 320712
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human CXCR2 transfected L1.2 cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: The 5E8/CXCR2 antibody is useful for immunofluorescent staining and flow cytometric analysis of CXCR2 expression. |
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| Application References(PubMed link indicates BioLegend citation) | Kyriakakis E, et al. 2011. J Leukoc Biol. 90:929. PubMed. |
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| Product Citations | Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed |
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| RRID | AB_492938 (BioLegend Cat. No. 320712) |
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| Structure | Seven transmembrane spanning, G protein-coupled receptor (CPCR), 67-70 kD, homodier or teterodimer with CXCR1 |
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| Distribution | Granulocytes, NK cells, subset of T cells, monocytes, endothelial cells, oligodendrocytes |
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| Function | Neutrophil activation, chemotaxis, angiogenesis |
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| Ligand/Receptor | IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), GRO-& alpha;,ß, γ (CXCL1, CXCL2, CXCL3) |
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| Cell Type | Granulocytes, NK cells, T cells, Monocytes, Endothelial cells, Oligodendrocytes, Neutrophils |
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| Biology Area | Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors, GPCR |
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| Antigen References | 1. Chuntharapai A, et al. 1994. J. Immunol. 153:5682. 2. Wilson S, et al. 2005. J. Biol. Chem. 280:28663. 3. Emadi S, et al. 2005. Blood 105:464. 4. Omari KM, et al. 2005. Brain 128:1003. 5. Juremalm M and Nilsson G. 2005. Chem. Immunol. Allergy 87:130. 6. Wolf M, et al. 1998. Eur. J. Immunol. 28:164. |
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| Gene ID | 3579 |
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| UniProt | View information about CD182 on UniProt.org |
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| Clone | 5E8/CXCR2 |
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| Regulatory Status | RUO |
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| Other Names | CD182, CXCR2, CD128b, IL-8RB, Type II IL-8 receptor |
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| Isotype | Mouse IgG1, κ |
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