Biolegend, 321116, Alexa Fluor® 647 anti-human CD206 (MMR) Antibody
100tests
Catalog Number: 321116
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Purified human mannose receptor |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | The 15-2 antibody blocks the interaction of MMR with its ligand, and inhibits mannose receptor-mediated degradation of t-PA by macrophages. Additional reported applications of this antibody (for the relevant formats) include: Western blotting1, blocking of ligand binding1,2, immunofluorescence3, and immunohistochemical staining of acetone-fixed frozen tissue sections1. The Utra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321149 and 321150). |
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| Application References(PubMed link indicates BioLegend citation) | Noorman F, et al. 1997. J. Leukocyte Biol. 61:63. (WB, IHC, Block) Barrett-Bergshoeff M, et al. 1997. Thromb Haemost. 77:718. (Block) Kato M, et al. 2007. J. Immunol. 179:6052. (IF) |
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| Product Citations | Mogilenko DA, et al. 2022. Immunol Cell Biol. 100:186. PubMed Liu J, et al. 2020. JCI Insight. 5:00. PubMed Bourdely P, et al. 2020. Immunity. 53(2):335-352. PubMed Sun L, et al. 2011. Am J Physiol Lung Cell Mol Physiol. 300:L341. PubMed Perera MR, et al. 2022. Int J Mol Sci. 23:. PubMed Xia W, et al. 2009. Blood. 113:438. PubMed |
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| RRID | AB_571881 (BioLegend Cat. No. 321116) |
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| Structure | Type I membrane protein, Pattern Recognition Receptor (PRR) family, C-type lectin superfamily, 162-175 kD |
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| Distribution | Macrophages, dendritic cells, hepatic and lymphatic endothelial cells |
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| Function | Pathogen binding, facilitate phagocytosis and endocytosis, macrophage activation and antigen presentation |
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| Ligand/Receptor | Mannose, fucose, N-acetyl glucosamine |
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| Cell Type | Dendritic cells, Endothelial cells, Macrophages |
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| Biology Area | Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Mason D, et al. Eds. 2002. Leukocyte Typing VII. Oxford University Press. p303 2. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501. 3. Apostolopoulos V and McKenzie IF. 2001. Curr. Mol. Med. 1:469. 4. Le Cabec V, et al. 2005. J. Leukocyte Biol. 77:934. 5. Barrett-Bergshoeff M, et al. 1997. Thromb. Haemostatis 77:718. |
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| Gene ID | 4360 |
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| UniProt | View information about CD206 on UniProt.org |
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| Clone | 15-2 |
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| Regulatory Status | RUO |
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| Other Names | MMR (macrophage mannose receptor), MR (mannose receptor), CD206, MRC1 |
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| Isotype | Mouse IgG1, κ |
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