Biolegend, 324210, Alexa Fluor® 488 anti-human CD326 (EpCAM) Antibody
100tests
Catalog Number: 324210
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | DU.4475 breast carcinoma |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested SB - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Additional reported applications (for the revelant formats) include: immunofluorescence, immunohistochemistry3, and spatial biology (IBEX)4,5. |
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| Additional Product Notes | Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH). |
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| Application References(PubMed link indicates BioLegend citation) | Lammers R, et al. 2002. Exp. Hematol. 30:537. Schultz LD, et al. 2010. P. Natl. Acad. Sci. USA 107:13022. PubMed Human Protein Atlas http://www.proteinatlas.org/ENSG00000119888/antibody (IHC) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Zabala M, et al. 2020. Cell Stem Cell. 27(2):284-299.e8. PubMed Qin XY, et al. 2023. Cell Death Dis. 14:358. PubMed Yu L, et al. 2020. Cell Stem Cell. 28(3):550-567.e12. PubMed Carraro G, et al. 2021. Nat Med. 27:806. PubMed Howden SE, et al. 2020. Cell Stem Cell. 28(4):671-684.e6. PubMed Pal D et al. 2017. eLife. 6 pii: e21615. PubMed Palkowitz AL, et al. 2021. Adv Healthc Mater. 10:e2100132. PubMed Pyo DH, et al. 2020. Cancer Biol Ther. 495:21. PubMed Morsing M, et al. 2020. Breast Cancer Res. 0.9875. PubMed Cai X, et al. 2014. PLoS One. 9:108942. PubMed Calandrini C, et al. 2020. Nat Commun. 11:1310. PubMed Gerdur ísberg ó, et al. 2019. Sci Rep. 9:14843. PubMed Inde Z, et al. 2021. Sci Adv. 7:. PubMed Sullivan NT, et al. 2022. STAR Protoc. 3:101367. PubMed Hu H et al. 2018. Cell. 175(6):1591-1606 . PubMed Chen D, et al. 2019. Cell Rep. 29:4568. PubMed Safaric Tepes P, et al. 2021. Elife. 10: . PubMed Cardenas JJ, et al. 2020. Methods Enzymol. 415:631. PubMed Gell JJ, et al. 2018. Stem Cell Res. 27:46. PubMed Noort V, et al. 2014. Cancer Res. 74:5690. PubMed Morris K, et al. 2014. PLoS One. 9:98656. PubMed Hong HK, et al. 2019. Oncol Rep. 42:2029. PubMed Hancock GV, et al. 2021. Stem Cell Res. 55:102493. PubMed Chen D et al. 2018. Cell reports. 25(13):3591-3602 . PubMed Vanslambrouck JM, et al. 2019. J Am Soc Nephrol. 30:1811. PubMed Chen D, et al. 2017. Biol Reprod. 97:850. PubMed Staudte S, et al. 2022. Cancers (Basel). 14:. PubMed Vanslambrouck JM, et al. 2022. Nat Commun. 13:5943. PubMed Cai X, et al. 2017. Neoplasia. 10.1016/j.neo.2017.03.005. PubMed Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed Horwitz K, et al. 2008. Proc Natl Acad Sci U S A. 105:5774. PubMed Chitiashvili T, et al. 2020. Nat Cell Biol. 22:1436. PubMed Sandlin CW, et al. 2022. PLoS One. 17:e0274091. PubMed Pandolfi EC, et al. 2022. Cell Rep Med. 3:100782. PubMed Mourcin F, et al. 2021. Immunity. . PubMed Catenacci D, et al. 2015. Gastroenterology. 149: 1794-1803.e4. PubMed Forbes TA, et al. 2018. Am J Hum Genet. 102:816. PubMed Jeong HO, et al. 2022. iScience. 25:105358. PubMed |
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| RRID | AB_756083 (BioLegend Cat. No. 324209) AB_756084 (BioLegend Cat. No. 324210) |
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| Structure | Type I transmembrane protein, contains six disulfide bridges, one THYRO domain, approximate molecular weight 40 kD. |
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| Distribution | Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis. |
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| Function | Homotypic calcium-independent cell adhesion. CD326 is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. |
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| Modification | Glycosylated. |
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| Cell Type | Embryonic Stem Cells, Epithelial cells |
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| Biology Area | Cell Biology, Immunology, Stem Cells |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Strnad J, et al. 1989. Cancer Res. 49:314. 2. Munz M, et al. 2004. Oncogene 23:5748. 3. Rao CG, et al. 2005. Int. J. Oncol. 27:49. |
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| Gene ID | 4072 |
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| UniProt | View information about CD326 on UniProt.org |
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| Clone | 9C4 |
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| Regulatory Status | RUO |
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| Other Names | Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, TROP1 |
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| Isotype | Mouse IgG2b, κ |
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