Biolegend, 324228, Alexa Fluor® 594 anti-human CD326 (EpCAM) Antibody
100microg
Catalog Number: 324228
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | DU.4475 breast carcinoma |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | IHC-P - Quality tested ICC - Verified SB - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, the suggested use of this reagent is 5.0 - 10 µg per mL. For immunocytochemistry, a concentration range of 2.5 - 10 μg per mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Green Laser (532 nm)/Yellow-Green Laser (561 nm) |
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| Application Notes | Additional reported applications (for the revelant formats) include: immunofluorescence, immunohistochemistry3, and spatial biology (IBEX)4,5. |
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| Additional Product Notes | Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH). |
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| Application References(PubMed link indicates BioLegend citation) | Lammers R, et al. 2002. Exp. Hematol. 30:537. Schultz LD, et al. 2010. P. Natl. Acad. Sci. USA 107:13022. PubMed Human Protein Atlas http://www.proteinatlas.org/ENSG00000119888/antibody (IHC) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Kenerson HL, et al. 2021. STAR Protocols. 2(2):100574. PubMed Roy J, et al. 2020. Theranostics. 10:5778. PubMed Sharma A, et al. 2020. Biotechnol Bioeng. 117:2540. PubMed Hough KP, et al. 2020. Methods. 27:177. PubMed Kure K, et al. 2020. Oncol Lett. 19:2286. PubMed |
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| RRID | AB_2563209 (BioLegend Cat. No. 324228) |
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| Structure | Type I transmembrane protein, contains six disulfide bridges, one THYRO domain, approximate molecular weight 40 kD. |
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| Distribution | Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis. |
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| Function | Homotypic calcium-independent cell adhesion. CD326 is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. |
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| Modification | Glycosylated. |
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| Cell Type | Embryonic Stem Cells, Epithelial cells |
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| Biology Area | Cell Biology, Immunology, Stem Cells |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Strnad J, et al. 1989. Cancer Res. 49:314. 2. Munz M, et al. 2004. Oncogene 23:5748. 3. Rao CG, et al. 2005. Int. J. Oncol. 27:49. |
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| Gene ID | 4072 |
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| UniProt | View information about CD326 on UniProt.org |
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| Clone | 9C4 |
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| Regulatory Status | RUO |
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| Other Names | Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, TROP1 |
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| Isotype | Mouse IgG2b, κ |
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