Biolegend, 328116, Alexa Fluor® 647 anti-human CD90 (Thy1) Antibody
100tests
Catalog Number: 328116
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| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Baboon, Cynomolgus, Pigtailed Macaque, Rhesus, Pig |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | HEL cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Clone 5E10 recognizes an epitope on Thy-1 independent of its glycosylation, but is abolished under reducing conditions.4 Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation1, and immunofluorescence3. |
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| Application References(PubMed link indicates BioLegend citation) | Craig W, et al. 1993. J. Exp. Med. 177:1331. (IP) Gundlach CW 4th, et al. 2011. Bioconjug. Chem. 22:1706. (Pig Reactivity) Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF) Bradley JE, et al. 2013. Lab Invest. 93:365. (Epitope) Donnenberg VS, et al. 2010. Cytometry B. Clin. Cytom. 5:287. (IHC) |
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| Product Citations | Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed Han J, et al. 2022. FEBS J. 289:417. PubMed Du Y, et al. 2012. Invest Ophthalmol Vis Sci. 53:1566. PubMed Robinson A, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:1115. PubMed de Sousa BM, et al. 2021. NPJ Regen Med. 6:80. PubMed |
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| RRID | AB_893439 (BioLegend Cat. No. 328115) AB_893430 (BioLegend Cat. No. 328116) |
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| Structure | 25-35 kD glycoprotein, Ig superfamily |
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| Distribution | Subset of CD34+ hematopoietic stem cells, subset of fetal thymocytes, subset of fetal liver cells, fibroblast, activated endothelial cells, neurons and some leukemia cell lines |
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| Function | Regulate hematopoiesis and neural cell growth, cell adhesion |
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| Ligand/Receptor | β3 integrin, β2 integrin |
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| Cell Type | Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Leukemia, Mesenchymal Stem Cells, Neurons, Thymocytes |
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| Biology Area | Immunology, Stem Cells |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. McKenzie JL, et al. 1981. J. Immunol. 126:843. 2. Avalos AM, et al. 2002. Biol. Res. 35:231. 3. Wetzel A, et al. 2004. J. Immunol. 172:3850. |
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| Gene ID | 7070 |
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| UniProt | View information about CD90 on UniProt.org |
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| Clone | 5E10 |
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| Regulatory Status | RUO |
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| Workshop | HCDM listed |
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| Other Names | Thy-1, Thy1 |
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| Isotype | Mouse IgG1, κ |
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