Biolegend, 328309, Alexa Fluor® 647 anti-human CD49a Antibody
25tests
Catalog Number: 328309
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| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Baboon, Cynomolgus, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human CTL line |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested SB - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications include: immunoprecipitation1, immunohistochemical staining1 of acetone-fixed frozen tissue sections, and spatial biology (IBEX)3,4. |
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| Additional Product Notes | Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH). |
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| Application References(PubMed link indicates BioLegend citation) | Hemler ME, et al. 1984. J.Immunol. 132:3011 Hemler ME, et al. 1985. J. Biol. Chem. 260:15246 Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Xu H, et al. 2019. Cell Stem Cell. 24:566. PubMed Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed Marquardt N, et al. 2019. Nat Commun. 10:3841. PubMed Miki K, et al. 2021. Nat Commun. 12:3596. PubMed Huang YL, et al. 2020. Elife. 9:00. PubMed Marquardt N, et al. 2015. J Immunol. 194:2467. PubMed |
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| RRID | AB_2129242 (BioLegend Cat. No. 328310) |
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| Structure | Integrin alpha chain family, Type I membrane protein, alpha chain of heterodimeric integrin receptor, 200 kD. Associates with CD29 to form VLA-1 complex |
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| Distribution | Activated T cells, monocytes, NK cells, smooth muscle cells, neuronal cells, fibroblasts, and mesenchymal cells |
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| Function | Adhesion, leukocyte migration |
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| Ligand/Receptor | With integrin β1 (CD29) forms receptor for laminin-1 and collagen IV |
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| Cell Type | Fibroblasts, Mesenchymal cells, Mesenchymal Stem Cells, Monocytes, NK cells, T cells |
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| Biology Area | Immunology, Stem Cells |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss Press. p122 Boiret N, et al. 2005. Exp. Hematol. 33:219 |
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| Gene ID | 3672 |
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| UniProt | View information about CD49a on UniProt.org |
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| Clone | TS2/7 |
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| Regulatory Status | RUO |
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| Other Names | α1 integrin, VLA-1 α chain, Integrin α1 chain, ITGA1 |
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| Isotype | Mouse IgG1, κ |
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