Biolegend, 330112, Alexa Fluor® 647 anti-human CD209 (DC-SIGN) Antibody
100tests
Catalog Number: 330112
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Extracellular domain of human DC-SIGN |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested ICC - Verified SB - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunohistochemistry on frozen tissue sections1 and spatial biology (IBEX)2,3. |
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| Additional Product Notes | Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH). |
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| Application References(PubMed link indicates BioLegend citation) | Granelli-Piperno A, et al. 2005. J Immunol. 175:4265. (IHC-F) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Surdziel E, et al. 2017. PLoS One.. 10.1371/journal.pone.0183679. PubMed Pagan JD, et al. 2018. Cell. 172:564. PubMed Prasad A, et al. 2017. Sci Rep. 7:40648. PubMed Olagnier D, et al. 2014. PLoS Pathog. 10:1004566. PubMed |
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| RRID | AB_1186094 (BioLegend Cat. No. 330111) AB_1186092 (BioLegend Cat. No. 330112) |
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| Distribution | Dendritic cells |
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| Cell Type | Dendritic cells |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Granelli-Piperno A, et al. 2005. J Immunol. 175:4265. |
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| Gene ID | 30835 |
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| UniProt | View information about CD209 on UniProt.org |
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| Clone | 9E9A8 |
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| Regulatory Status | RUO |
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| Other Names | Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin |
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| Isotype | Mouse IgG2a, κ |
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