Biolegend, 330932, Alexa Fluor® 488 anti-human CD58 (LFA-3) Antibody
100tests
Catalog Number: 330932
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| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Baboon, Cynomolgus, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human cytolytic T cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Additional reported applications include: immunoprecipitation1, inhibition of cytolytic activity1, augment of IL-1 release by TE cells2 |
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| Application References(PubMed link indicates BioLegend citation) | Sanchez-Madrid F, et al. 1982. Proc. Nati Acad. Sci. USA. 79:7489 Le PT, et al. 1990. J. Immunol. 144:4541 |
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| RRID | AB_3683345 (BioLegend Cat. No. 330931) AB_3683345 (BioLegend Cat. No. 330932) |
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| Structure | Immunoglobulin superfamily member, 45-70 kD cell surface protein. Alternative splicing gives rise to transmembrane and glycosylphosphatidylinositol (GPI)-anchored form on cell surface. |
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| Distribution | Expressed on both hematopoietic and non-hematopoietic cells including B cells, T cells, monocytes, erythrocytes, endothelial cells, epithelial cells, and fibroblasts. High levels are observed on memory T cells and dendritic cells. |
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| Function | Mediates adhesion between various cell types (target cells and cytotoxic cells, APC and T cells, thymocytes and thymic epithelia). |
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| Ligand/Receptor | CD2 |
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| Cell Type | B cells, Dendritic cells |
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| Biology Area | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Springer TA, et al. 1987. Annu. Rev. Immunol. 5:223. 2. Dustin ML, et al. 1987. Nature 329:846. 3. Arulanandam AR, et al. 1994. J. Exp. Med. 180:1861. 4. Sanders ME, et al. 1988. J. Immunol. 140:1401. |
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| Gene ID | 965 |
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| UniProt | View information about CD58 on UniProt.org |
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| Clone | TS2/9 |
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| Regulatory Status | RUO |
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| Other Names | Lymphocyte function-associated antigen 3, LFA-3 |
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| Isotype | Mouse IgG1, κ |
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