Biolegend, 333812, Alexa Fluor® 488 anti-human CD68 Antibody
100tests
Catalog Number: 333812
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser |
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| Blue Laser (488 nm) |
| Application Notes | Additional reported application: immunohistochemical staining of frozen tissue sections. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. |
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| Application References(PubMed link indicates BioLegend citation) | Doussis IA, et al. 1993. J. Clin. Pathol. 46:334. Davey FR, et al. 1988. J. Clin. Pathol. 41:753. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed |
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| Product Citations | Li M, et al. 2023. Front Immunol. 14:1087923. PubMed Smith MH, et al. 2023. Nat Immunol. . PubMed Zhao P, et al. 2022. MAbs. 14:2107971. PubMed Managò A, et al. 2019. Nat Commun. 10:4116. PubMed McAvoy M, et al. 2020. Front Neurol. 0.905555556. PubMed Ohmatsu H, et al. 2017. Oncoimmunology. 6:e1181237. PubMed Li M, et al. 2020. Nat Commun. 4051:11. PubMed Gage BK, et al. 2020. Cell Stem Cell. 27(2):254-269.e9. PubMed |
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| RRID | AB_2074833 (BioLegend Cat. No. 333811) AB_2074832 (BioLegend Cat. No. 333812) |
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| Structure | Sialomucin family, 110 kD |
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| Distribution | Monocytes/macrophages, dendritic cells, granulocytes, subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, endothelial cells |
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| Cell Type | B cells, Dendritic cells, Endothelial cells, Fibroblasts, Granulocytes, Hematopoietic stem and progenitors, Macrophages, Monocytes, T cells |
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| Biology Area | Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Holness CL and Simmons DL. 1993. Blood 81:1607. 2. Gottfried E, et al. 2008. Scand. J. Immunol. 67:453. 3. Hameed A, et al. 1994. Hum. Pathol. 25:872. |
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| Gene ID | 968 |
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| UniProt | View information about CD68 on UniProt.org |
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| Clone | Y1/82A |
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| Regulatory Status | RUO |
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| Workshop | VI MR23 |
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| Other Names | Macrosialin |
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| Isotype | Mouse IgG2b, κ |
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