Biolegend, 334210, Alexa Fluor® 647 anti-human CD202b (Tie2/Tek) Antibody
100tests
Catalog Number: 334210
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Recombinant extracellular domain of human Tie2 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications include: immunoprecipitation, Wstern blot, immunohistochemical staining of frozen tissue sections and ELISA |
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| Application References(PubMed link indicates BioLegend citation) | Peters KG, et al. 1998. Br. J. Cancer. 77:51. Wong AL, et al. 1997. Circ. Res. 81:567. Lin P, et al. 1998. P. Natl. Acad. Sci. USA 95:8829. |
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| Product Citations | Kato D, et al. 2020. Elife. :9. PubMed Sakai D, et al. 2018. JOR Spine. 1:e1018. PubMed Rogacev K, et al. 2015. Nephrol Dial Transplant. 30:143. PubMed Khan OF, et al. 2018. Sci Adv. 4:eaar8409. PubMed Wangler S, et al. 2019. Spine (Phila Pa 1976). 44:1613. PubMed Shlamkovich T, et al. 2020. BMC Biology. 16(1):92. PubMed |
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| RRID | AB_2255984 (BioLegend Cat. No. 334209) AB_2203206 (BioLegend Cat. No. 334210) |
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| Structure | 145 kD type I transmembrane protein, receptor tyrosine kinase (RTK) family |
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| Distribution | Endothelial cells, subsets of hematopoietic stem cells and and a subset of monocytes |
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| Function | Angiogenesis, hemotopoiesis, monocytes chemotaxis |
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| Ligand/Receptor | Angiopoietin-1 (Ang-1), Ang-2, and Ang-4 |
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| Cell Type | Endothelial cells, Hematopoietic stem and progenitors, Monocytes |
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| Biology Area | Angiogenesis, Cell Biology, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1.De Palma M, et al. 2005. Cancer Cell. 8:211 2.Shaw JP, et al. 2004. Blood Cells Mol. Dis. 32:168 3. Hsu HC, et al. 2000. Blood. 96:3757 4. Arai F, et al. 2004. Cell. 118:149 |
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| Gene ID | 7010 |
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| UniProt | View information about CD202b on UniProt.org |
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| Clone | 33.1 (Ab33) |
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| Regulatory Status | RUO |
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| Other Names | Tie2, TEK |
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| Isotype | Mouse IgG1, κ |
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