Biolegend, 334312, Alexa Fluor® 647 anti-human CD40 Antibody
100tests
Catalog Number: 334312
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| Verified Reactivity | Human, Cynomolgus, Rhesus |
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| Reported Reactivity | Baboon, Chimpanzee, Squirrel Monkey |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation1, partial inhibition of CD40 binding to CD40L3, and B cell rescue from apoptosis1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. |
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| Application References(PubMed link indicates BioLegend citation) | Schlossman SF, et al. 1995. ed. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Pound JD, et al. 1999. Int. Immunol. 11:11. Shey MS, et al. 2014. J Immunol. 192:4833. PubMed Sondergaard JN, et al. 2014. Mol Immunol. 59:180. PubMed Saiki O, et al. 1995. J Clin. Invest. 2: 510-4 (Costim) |
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| Product Citations | Charles N, et al. 2010. Nat Med. 701:16. PubMed Heninger A, et al. 2013. Diabetes. 62:2059. PubMed |
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| RRID | AB_1186051 (BioLegend Cat. No. 334311) AB_1186029 (BioLegend Cat. No. 334312) |
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| Structure | TNFR superfamily, type I glycoprotein, 48 kD |
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| Distribution | B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts |
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| Function | B cell differentiation, costimulation, isotype class-switching, rescues B cells from apoptosis |
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| Ligand/Receptor | CD154 (CD40 ligand) |
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| Cell Type | B cells, Dendritic cells, Endothelial cells, Fibroblasts, Macrophages |
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| Biology Area | Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881. 2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591. |
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| Gene ID | 958 |
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| UniProt | View information about CD40 on UniProt.org |
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| Clone | 5C3 |
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| Regulatory Status | RUO |
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| Workshop | V CD40.4 |
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| Other Names | BP50, TNFRSF5 |
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| Isotype | Mouse IgG1, κ |
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