Biolegend, 338830, Alexa Fluor® 488 anti-human CD44 Antibody
100tests
Catalog Number: 338830
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| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Baboon, Cynomolgus, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Normal human PBL |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Blue Laser (488 nm) |
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| Application References(PubMed link indicates BioLegend citation) | Kishimoto T, et al. eds. 1997 Leucocyte Typing VI:White Cell Differentiation Antigen. Garland Publishing Inc. |
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| Product Citations | Hamester F, et al. 2022. Cells. 11:. PubMed |
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| RRID | AB_2814239 (BioLegend Cat. No. 338829) AB_2819968 (BioLegend Cat. No. 338830) |
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| Structure | Variable splicing of CD44 gene generates many CD44 isoforms, 85 kD |
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| Distribution | All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells |
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| Function | Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM |
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| Ligand/Receptor | Hyaluronan, MIP-1β, fibronectin, collagen |
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| Cell Type | Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs |
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| Biology Area | Cell Adhesion, Cell Biology, Immunology, Stem Cells |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Haynes BF, et al. 1991. Cancer Cells 3:347. 3. Goldstein LA, et al. 1989. Cell 56:1063. 4. Mikecz K, et al. 1995. Nat. Med. 1:558. |
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| Gene ID | 960 |
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| UniProt | View information about CD44 on UniProt.org |
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| Clone | BJ18 |
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| Regulatory Status | RUO |
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| Workshop | VI A034 |
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| Other Names | Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24 |
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| Isotype | Mouse IgG1, κ |
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