Biolegend, 343507, Alexa Fluor® 647 anti-human CD34 Antibody
25tests
Catalog Number: 343507
Send an RFQ.
Category
Loading…
| Verified Reactivity | Human |
|---|---|
| Reported Reactivity | Cynomolgus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Excitation Laser | Red Laser (633 nm) |
|---|
| Application Notes | The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6. |
|---|
| Application References(PubMed link indicates BioLegend citation) | Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press. Felschow DM, et al. 2001. Blood 97:3768. Rudin CE, et al. 1997. Br. J. Haematol. 97:488. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC) Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF) |
|---|
| Product Citations | Smith MH, et al. 2023. Nat Immunol. . PubMed Rothe K, et al. 2020. Cell Stem Cell. 27(1):110-124.e9. PubMed Knapp D, et al. 2017. Blood. 129(3):307-318. PubMed Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. PubMed Zhang HF, et al. 2021. Cancer Discov. 11:2884. PubMed Mendes R, et al. 2016. Infect Immun . 84: 2141 - 2148. PubMed Salik B, et al. 2020. Cancer Cell. 38(2):263-278.e6. PubMed Bade RM, et al. 2021. Molecular Oncology. . PubMed |
|---|
| RRID | AB_1877134 (BioLegend Cat. No. 343507) AB_1877133 (BioLegend Cat. No. 343508) |
|---|
| Structure | 105-120 kD single chain mucin-like glycoprotein |
|---|
| Distribution | Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts |
|---|
| Function | Cell adhesion |
|---|
| Ligand/Receptor | L-selectin, E-selectin |
|---|
| Cell Type | Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors |
|---|
| Biology Area | Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells |
|---|
| Molecular Family | CD Molecules |
|---|
| Antigen References | 1. Krause DS, et al. 1996. Blood 87:1. 2. Puri KD, et al. 1995. J. Cell Biol. 131:261. 3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey. |
|---|
| Gene ID | 947 |
|---|
| UniProt | View information about CD34 on UniProt.org |
|---|
| Clone | 581 |
|---|
| Regulatory Status | RUO |
|---|
| Workshop | V MA27 |
|---|
| Other Names | Gp105-120, My10 |
|---|
| Isotype | Mouse IgG1, κ |
|---|
