Biolegend, 343518, Alexa Fluor® 488 anti-human CD34 Antibody
100tests
Catalog Number: 343518
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| Verified Reactivity | Human |
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| Reported Reactivity | Cynomolgus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6. |
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| Application References(PubMed link indicates BioLegend citation) | Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press. Felschow DM, et al. 2001. Blood 97:3768. Rudin CE, et al. 1997. Br. J. Haematol. 97:488. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC) Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF) |
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| Product Citations | Voit RA, et al. 2023. Nat Immunol. 24:69. PubMed Borrelli MR, et al. 2020. Stem Cells Transl Med. 1.339583333. PubMed Dong Z, et al. 2013. PLoS One. 18:80364. PubMed Galán-Díez M, et al. 2022. Cancer Discov. . PubMed Joyce CE, et al. 2019. J Clin Invest. 130:. PubMed Khajuria RK et al. 2018. Cell. 173(1):90-103 . PubMed Li J, et al. 2017. Toxicology. 378:17. PubMed Deleon NMD, et al. 2021. J Tissue Eng Regen Med. 15:1105. PubMed Bianchi A, et al. 2022. Genome Biol. 23:229. PubMed |
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| RRID | AB_1937204 (BioLegend Cat. No. 343517) AB_1937203 (BioLegend Cat. No. 343518) |
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| Structure | 105-120 kD single chain mucin-like glycoprotein |
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| Distribution | Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts |
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| Function | Cell adhesion |
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| Ligand/Receptor | L-selectin, E-selectin |
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| Cell Type | Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors |
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| Biology Area | Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Krause DS, et al. 1996. Blood 87:1. 2. Puri KD, et al. 1995. J. Cell Biol. 131:261. 3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey. |
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| Gene ID | 947 |
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| UniProt | View information about CD34 on UniProt.org |
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| Clone | 581 |
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| Regulatory Status | RUO |
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| Workshop | V MA27 |
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| Other Names | Gp105-120, My10 |
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| Isotype | Mouse IgG1, κ |
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