Biolegend, 343930, Alexa Fluor® 647 anti-human CD166 Antibody
100tests
Catalog Number: 343930
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| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Baboon, Cynomolgus, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Cultured human thymic epithelial cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections and immunofluorescence.1 |
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| Application References(PubMed link indicates BioLegend citation) | Pretzel D, et al. 2011. Arthritis Res. Ther. 13:R64. (IHC, IF, FC) |
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| RRID | AB_3083329 (BioLegend Cat. No. 343929) AB_3083329 (BioLegend Cat. No. 343930) |
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| Structure | A type I transmembrane glycoprotein belonging to the Ig superfamily of proteins. |
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| Distribution | CD166 is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, and neurons. |
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| Function | Play an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. |
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| Ligand/Receptor | CD6 |
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| Cell Type | Epithelial cells, Fibroblasts, Mesenchymal Stem Cells, Monocytes, Neurons, T cells |
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| Biology Area | Immunology, Stem Cells |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Aruffo A, et al. 1997. Immunol Today. 18(10):498 2. Patel DD, et al. 1995. J. Exp. Med. 181:2213 3. Bowen MA, et al. 1995. J. Exp. Med. 181:1563 4. Horst D, et al. 2009. Cancer Invest. 22:1 |
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| Gene ID | 214 |
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| UniProt | View information about CD166 on UniProt.org |
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| Clone | 3A6 |
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| Regulatory Status | RUO |
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| Workshop | HCDM listed |
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| Other Names | CD6 ligand, Activated Leukocyte Cell Adhesion Molecule (ALCAM) |
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| Isotype | Mouse IgG1, κ |
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