Biolegend, 344042, Alexa Fluor® 700 anti-human CD73 (Ecto-5'-nucleotidase) Antibody
100tests
Catalog Number: 344042
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| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Baboon |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include:immunofluorescence3. Clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4. |
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| Application References(PubMed link indicates BioLegend citation) | Nakamura T, et al. 1993. J. Immunol. 151:6933. Liao J, et al. 2011. J Endod. 37:1217. PubMed Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF) Terp MG, et al. 2013. J Immunol. 191: 4165-73 (Block) |
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| Product Citations | Wilson A, et al. 2023. F1000Res. 1280:11. PubMed |
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| RRID | AB_2927998 (BioLegend Cat. No. 344041) AB_2927998 (BioLegend Cat. No. 344042) |
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| Structure | GPI-linked 5'-nucleotidase, 70 kD |
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| Distribution | Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells |
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| Function | Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells |
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| Cell Type | B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs |
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| Biology Area | Costimulatory Molecules, Immunology, Stem Cells |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication. 2. Airas L and Jalkanen S, et al. 1996. Blood 88:1755. 3. Gutensohn W, et al. 1995. Cell Immunol. 161:213. 4. Airas L, et al. 1995. J. Exp. Med. 182:1603. |
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| Gene ID | 4907 |
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| UniProt | View information about CD73 on UniProt.org |
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| Clone | AD2 |
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| Regulatory Status | RUO |
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| Workshop | V B-CD73.3 |
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| Other Names | Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT |
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| Isotype | Mouse IgG1, κ |
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