Biolegend, 346005, Alexa Fluor® 647 anti-human CD169 (Sialoadhesin, Siglec-1) Antibody
25tests
Catalog Number: 346005
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human Rhinovirus (HRV14) infected, monocyte derived-DCs |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: Western blotting and inhibition of erythrocyte-rosetting with cells expressing CD169. |
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| Application References(PubMed link indicates BioLegend citation) | Kirchberger S, et al. 2005. J. Immunol. 175:1145. Schrauf C, et al. 2009. J. Immunol. 183:4440. |
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| Product Citations | Preglej T, et al. 2023. Front Immunol. 14:1096096. PubMed Jalloh S, et al. 2022. PLoS Pathog. 18:e1010479. PubMed Li C, et al. 2015. PLoS One. 10: e0141817. PubMed Rose T, et al. 2016. RMD Open. 2: e000292. PubMed |
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| RRID | AB_10900414 (BioLegend Cat. No. 346005) AB_10897945 (BioLegend Cat. No. 346006) |
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| Structure | Type I single membrane-spanning lectin, 1709 amino acids, 210 kD, belongs to the immunoglobulin superfamily |
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| Distribution | Macrophages, dendritic cells |
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| Function | Adhesion molecule |
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| Interaction | α-2,3-linked sialic acid |
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| Ligand/Receptor | CD227, CD43 and CD206 |
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| Bioactivity | Mediates sialic-acid dependent binding to granulocytes, monocytes, NK, B and T cells. |
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| Cell Type | B cells, Dendritic cells, Macrophages |
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| Biology Area | Cell Adhesion, Cell Biology, Immunology, Innate Immunity |
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| Molecular Family | Adhesion Molecules, CD Molecules, Siglec Molecules |
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| Antigen References | 1. Xiong YS, et al. 2009. Clin. Biochem. 42:1057. 2. Varki A, et al. 2009. Glycoconj J. 26:231. 3. Rempel H, et al. 2008. PLoS One. 3:e1967. 4. Crocker PR, et al. 2001. Trends Immunol. 22:337. 5. Hartnell A, et al. 2001. Blood 97:288. |
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| Gene ID | 6614 |
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| UniProt | View information about CD169 on UniProt.org |
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| Clone | 7-239 |
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| Regulatory Status | RUO |
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| Other Names | Sialic acid binding Ig-like lectin 1 (Siglec-1) |
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| Isotype | Mouse IgG1, κ |
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