Biolegend, 346007, APC anti-human CD169 (Sialoadhesin, Siglec-1) Antibody
25tests
Catalog Number: 346007
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human Rhinovirus (HRV14) infected, monocyte derived-DCs |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. |
| Excitation Laser | Red Laser (633 nm) |
| Application Notes |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
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| Additional reported applications (for the relevant formats) include: Western blotting and inhibition of erythrocyte-rosetting with cells expressing CD169. |
| Application References(PubMed link indicates BioLegend citation) | Kirchberger S, et al. 2005. J. Immunol. 175:1145. Schrauf C, et al. 2009. J. Immunol. 183:4440. |
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| Product Citations | Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed Lertjuthaporn S, et al. 2022. Pathogens. 11: . PubMed Aravantinou M, et al. 2016. PLoS One. 11: 0161730. PubMed Evren E, et al. 2020. Immunity. 54(2):259-275.e7. PubMed Perot BP, et al. 2020. Front Cell Infect Microbiol. 10:70. PubMed Obradovic A, et al. 2021. Cell. 184(11):2988-3005.e16. PubMed Rai MA, et al. 2020. Retrovirology. 17:35. PubMed Sefik E, et al. 2021. Nat Biotechnol. . PubMed Gadd V, et al. 2016. PLoS One. 11: 0157771. PubMed Soberanes S et al. 2018. Cell metabolism. 29(2):335-347 . PubMed Jobe O, et al. 2016. J Leukoc Biol. 99: 1089 - 1106. PubMed |
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| RRID | AB_11150773 (BioLegend Cat. No. 346007) AB_11147948 (BioLegend Cat. No. 346008) |
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| Structure | Type I single membrane-spanning lectin, 1709 amino acids, 210 kD, belongs to the immunoglobulin superfamily |
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| Distribution | Macrophages, dendritic cells |
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| Function | Adhesion molecule |
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| Interaction | α-2,3-linked sialic acid |
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| Ligand/Receptor | CD227, CD43 and CD206 |
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| Bioactivity | Mediates sialic-acid dependent binding to granulocytes, monocytes, NK, B and T cells. |
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| Cell Type | B cells, Dendritic cells, Macrophages |
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| Biology Area | Cell Adhesion, Cell Biology, Immunology, Innate Immunity |
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| Molecular Family | Adhesion Molecules, CD Molecules, Siglec Molecules |
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| Antigen References | 1. Xiong YS, et al. 2009. Clin. Biochem. 42:1057. 2. Varki A, et al. 2009. Glycoconj J. 26:231. 3. Rempel H, et al. 2008. PLoS One. 3:e1967. 4. Crocker PR, et al. 2001. Trends Immunol. 22:337. 5. Hartnell A, et al. 2001. Blood 97:288. |
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| Gene ID | 6614 |
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| UniProt | View information about CD169 on UniProt.org |
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| Clone | 7-239 |
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| Regulatory Status | RUO |
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| Other Names | Sialic acid binding Ig-like lectin 1 (Siglec-1) |
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| Isotype | Mouse IgG1, κ |
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