Biolegend, 346203, Alexa Fluor® 647 anti-human Basophils (2D7) Antibody
25tests
Catalog Number: 346203
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Category
| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Lysates of purified basophils |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications for the relevant formats include: immunofluorescence staining7, and immunohistochemistry of acetone-fixed frozen sections1 and formalin-fixed paraffin-embedded sections1-6. |
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| Application References(PubMed link indicates BioLegend citation) | Kepley CL, et al. 1995. J. Immunol. 154:6548. (IHC) Irani AM, et al. 1998. J. Allergy Clin. Immunol. 1998 101:354. (IHC) Agis H, et al. 2006. J. Clin. Pathol. 59:396. (IHC) Plager DA, et al. 2006. J. Allergy Clin. Immunol. 117:626. (IHC) Sperr WR, et al. 2001. Blood 98:2200. (IHC) Aichberger KJ, et al. 2006. Blood 108:3538. (IHC) Kepley CL, et al. 1998. J. Allergy Clin. Immun. 102:304. (IF) |
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| RRID | AB_1967142 (BioLegend Cat. No. 346204) |
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| Distribution | Basophils. |
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| Cell Type | Basophils |
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| Biology Area | Immunology, Innate Immunity |
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| Gene ID | NA |
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| UniProt | View information about Basophil on UniProt.org |
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| Clone | Basophil/2D7 |
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| Regulatory Status | RUO |
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| Isotype | Mouse IgG1, κ |
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