Biolegend, 347316, Alexa Fluor® 647 anti-human CD115 (CSF-1R) Antibody
100tests
Catalog Number: 347316
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| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Baboon, Cynomolgus, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | C-fms transduced Kirsten strain murine sarcoma virus transformed NRK cells. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1151. |
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| Application References(PubMed link indicates BioLegend citation) | Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed |
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| RRID | AB_2687249 (BioLegend Cat. No. 347315) AB_2687250 (BioLegend Cat. No. 347316) |
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| Structure | Single-pass type I membrane protein and is 150 kD. |
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| Distribution | Monocytes/macrophages, plasmacytoid and conventional dendritic cells, and osteoclasts. |
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| Ligand/Receptor | Macrophage Colony Stimulating Factor (M-CSF) and IL-34. |
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| Cell Type | Dendritic cells, Macrophages, Monocytes, Osteoclasts |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors |
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| Antigen References | 1. Sherr CJ, et al. 1989. Blood 73:1786 2. Roussel MF, et al. 1991. Nature 353:361. 3. Roussel MF, et al. 1989 P. Natl. Acad. Sci. USA 86:7924. |
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| Gene ID | 1436 |
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| UniProt | View information about CD115 on UniProt.org |
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| Clone | 9-4D2-1E4 |
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| Regulatory Status | RUO |
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| Workshop | V MA199 |
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| Other Names | M-CSFR,CSF-1R, FMS, CSF-1 Receptor |
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| Isotype | Rat IgG1, κ |
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