Biolegend, 348005, Alexa Fluor® 647 anti-human Granulysin Antibody
25tests
Catalog Number: 348005
Send an RFQ.
Category
Loading…
| Verified Reactivity | Human |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Recombinant 9 kD granulysin |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality testedIHC - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Excitation Laser | Red Laser (633 nm) |
|---|
| Application Notes | Clone DH2 recognizes both the 9 kD and 15 kD isoforms of Granulysin.1ELISA Capture: To measure human granulysin, this antibody can be used as a capture antibody in sandwich ELISA format and paired with the biotinylated DH10 antibody (Cat. No. 526103) as the detection antibody. Recombinant Human Granulysin (ELISA Std.) (Cat. No. 552109) can be used as the protein standard.Flow Cytometry: The fluorochrome-labeled DH2 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify granulysin-producing cells within mixed cell populations.Note: For testing human granulysin in serum, plasma or cell culture supernatant, LEGEND MAX™ Human Granulysin ELISA Kit with Pre-coated Plates (Cat. No. 438007 & 438008) are specially developed and recommended. |
|---|
| Application References(PubMed link indicates BioLegend citation) | Hanson DA, et al. 1999. Mol. Immunol. 36:413. Spada FM, et al. 2000. J. Exp. Med. 191:937. |
|---|
| Product Citations | Singh KS, et al. 2021. Nature. 589:597. PubMed |
|---|
| RRID | AB_2110110 (BioLegend Cat. No. 348006) |
|---|
| Structure | Saposin-like protein family of lipid binding protein, 9 kD and 15 kD |
|---|
| Distribution | NK cells, cytolytic T cells |
|---|
| Function | Cytolytic against bacteria, fungi, parasite and tumor cells; chemoattract inflammatory cells |
|---|
| Cell Type | NK cells, T cells |
|---|
| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity |
|---|
| Molecular Family | Cytokines/Chemokines |
|---|
| Antigen References | 1. Krensky AM. 2000. Biochem. Pharmacol. 59:317. 2. Deng A, et al. 2005. J. Immunol. 174:5243. 3. Kaspar AA, et al. 2001. J. Immunol. 167:350. 4. Krensky AM and Clayberger C. 2009. Tissue Antigens 73:193. |
|---|
| Gene ID | 10578 |
|---|
| UniProt | View information about Granulysin on UniProt.org |
|---|
| Clone | DH2 |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | GNLY, 519, LAG2, NKG5, LAG-2, D2S69E, TLA519 |
|---|
| Isotype | Mouse IgG1, κ |
|---|
