Biolegend, 348514, Alexa Fluor® 647 anti-human CD144 (VE-Cadherin) Antibody
100tests
Catalog Number: 348514
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested IHC-P - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Clone BV9 has been shown to block VE-cadherin, causing a redistribution of VE-cadherin away from intracellular junctions.6 This clone binds to EC3-EC4 region in the extracellular domain of human VE-cadherin.7 Additional reported applications (for the relevant formats) include: Western Blotting1,2, immunofluorescence microscopy1,3, immunoprecipitation1,4, blocking angiogenesis in vitro4,5, inhibiting VE-cadherin reorganization4, and inducing endothelial cell apoptosis4. |
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| Application References(PubMed link indicates BioLegend citation) | Almagro S, et al. 2010. Mol. Cell Biol. 30:1703. (WB, IF, IP) Zhang F, et al. 2004. J. Biol. Chem. 279:11760. (WB) Iurlaro M, et al. 2004. Am. J. Pathol. 165:181. (IF) Corada M, et al. 2001. Blood 97:1679. (IP, Block) Kooistra M, et al. 2005. FEBS 579:4966. (Block) Corada M, et al. 2001. Blood 97:1679. (Block) Bouillet L, et al. 2013. Laboratory Investigation 93:1194-11202. |
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| RRID | AB_2563669 (BioLegend Cat. No. 348513) AB_2563670 (BioLegend Cat. No. 348514) |
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| Structure | Member of the cadherin family; calcium-dependent transmembrane cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats and a highly conserved cytoplasmic tail region |
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| Distribution | Endothelial cells, hematopoietic stem cells, perineurial cells |
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| Function | Mediates calcium-dependent homophilic cell adhesion, plays fundamental roles in microvascular permeability and in the morphogenic and proliferative events associated with angiogenesis |
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| Ligand/Receptor | VE-cadherin |
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| Cell Type | Endothelial cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells |
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| Biology Area | Angiogenesis, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Stem Cells |
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| Molecular Family | Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase |
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| Antigen References | 1. Taddei A, et al. 2008. Nat. Cell Biol. 10:923. 2. Gavard J, et al. 2006. Nat. Cell Biol. 8:1223. 3. Kim I, et al. 2005. Blood 106:903. 4. Suzuki S, et al. 1991. Cell Regul. 2:261. |
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| Gene ID | 1003 |
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| UniProt | View information about CD144 on UniProt.org |
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| Clone | BV9 |
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| Regulatory Status | RUO |
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| Other Names | VE-Cadherin, cadherin-5, CDH5 |
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| Isotype | Mouse IgG2a, κ |
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