Biolegend, 349129, Alexa Fluor® 647 anti-human CD235a (Glycophorin A) Antibody
25tests
Catalog Number: 349129
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application References(PubMed link indicates BioLegend citation) | Mason D, et al. Eds. 2002. Leucocyte Typing VII:White Cell Differentiation Antigens. Oxford University Press. (FC) |
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| RRID | AB_2936725 (BioLegend Cat. No. 349129) AB_2936725 (BioLegend Cat. No. 349130) |
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| Structure | Member of the glycophorin A family; type I protein with a molecular weight of 10 kD; present in the membrane as homodimer |
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| Distribution | Erythrocytes and erythroid precursors |
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| Function | Possible inhibitor of hemagglutination and hemolysis; carries the antigen determinants for the MNS blood groups |
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| Ligand/Receptor | Siglec 5, erythrocyte binding antigen (EBA-175) of P. falciparum, receptor for the influenza virus, hepatitis A virus |
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| Cell Type | Erythrocytes |
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| Biology Area | Cell Adhesion, Cell Biology, Immunology |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Reid ME. 2009. Immunohematology 25:95. 2. Palacajornsuk P. 2006. Immunohematology 22:171. 3. Pasvol G. 2003. Trends Parasitol. 19:430. 4. Takakuwa Y. 2001. Curr. Opin. Hematol. 8:80. |
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| Gene ID | 2993 |
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| UniProt | View information about CD235a on UniProt.org |
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| Clone | HI264 |
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| Regulatory Status | RUO |
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| Workshop | VII 70312 |
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| Other Names | PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, Glycophorin A, GYPA |
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| Isotype | Mouse IgG2a, κ |
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