Biolegend, 349517, Alexa Fluor® 700 anti-human CD81 (TAPA-1) Antibody
25tests
Catalog Number: 349517
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| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Baboon, Cynomolgus, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human OCI-LY8 cell line |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: Western Blotting3 and immunoprecipitation2,3. |
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| Application References(PubMed link indicates BioLegend citation) | Van Zelm MC, et al. 2010. J. Clin. Invest. 120:1265. Oren R, et al. 1990. Mol. Cell. Biol. 8:4007. (IP) Clark K, et al. 2004. J. Biol. Chem. 279(19):19401. (IP, WB) Mochida K, et al. 2008. J. Virol. 13:6711. Rappa G, et al. 2014. Mol Cancer Res. 12:1840. PubMed |
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| Product Citations | Newman LA, et al. 2021. Cells. 10:. PubMed |
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| RRID | AB_2687128 (BioLegend Cat. No. 349517) AB_2687129 (BioLegend Cat. No. 349518) |
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| Structure | 26 kD, type III transmembrane protein, member of the TM4SF tetraspanin family. Complexed with CD82, CD19, CD21, or CD4, CD8. |
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| Distribution | T cells, NK, monocytes, B cells, endothelial and epithelial cells, low on granulocytes. |
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| Function | Regulates cell activation and growth and cell aggregation. |
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| Cell Type | B cells, Endothelial cells, Epithelial cells, Monocytes, Neural Stem Cells, NK cells, T cells |
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| Biology Area | Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Transcription Factors |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | Van Zelm MC, et al. 2010. J. Clin. Invest. 120:1265. Fearon D, et al. 1995. Annu. Rev. Immunol. 13:127. Wright M, et al. 1994. Immunol. Today 15:588. |
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| Gene ID | 975 |
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| UniProt | View information about CD81 on UniProt.org |
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| Clone | 5A6 |
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| Regulatory Status | RUO |
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| Other Names | S5.7, CVID6, TSPAN28 |
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| Isotype | Mouse IgG1, κ |
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