Biolegend, 350018, Alexa Fluor® 647 anti-human CD134 (OX40) Antibody
100tests
Catalog Number: 350018
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| Verified Reactivity | Human |
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| Reported Reactivity | Chimpanzee |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | HTLV 1-transformed HUT 102 cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, immunohistochemical staining2,3of paraffin embedded7 and frozen tissue sections, ELISA4, and functional assay5. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team). |
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| Application References(PubMed link indicates BioLegend citation) | Latza U, et al. 1994. Eur. J. Immunol. 24:677. (WB, IP) Durkop H, et al. 1995. Brit. J. Haematol. 91:927. (IHC) Durkop H, et al. 1997. Brit. J. Haematol. 98:863. (IHC) Willett B, et al. 2007. J. Virol. 81:9665. (ELISA) Li M and Zhang Y. et al. 2005. Cell. Mol. Immunol. 2:467. (FA) Gloviczki ML, et al. 2012. Clin. J. Am. Soc. Nephrol. 8:546. PubMed Domingos PL, et al. 2012. An. Bras. Dermatol. 87:851. (IHC) |
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| Product Citations | Siegemund M, et al. 2021. Front Pharmacol. 12:759337. PubMed Geuijen C, et al. 2021. Nat Commun. 12:4445. PubMed Kuo HH, et al. 2018. Immunity. 48:1183. PubMed Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed Nguyen J, et al. 2021. Mol Syst Biol. 17:e10560. PubMed |
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| RRID | AB_2571937 (BioLegend Cat. No. 350017) AB_2571938 (BioLegend Cat. No. 350018) |
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| Structure | 50 kD, TNF receptor family |
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| Distribution | Activated T cells |
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| Function | Receptor for OX40 ligand, co-stimulatory signal for T cell proliferation and survival. |
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| Ligand/Receptor | OX40L (CD252) |
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| Cell Type | T cells, Tregs |
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| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immunology |
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| Molecular Family | CD Molecules, Immune Checkpoint Receptors |
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| Antigen References | 1. Smith CA, et al. 1994. Cell. 76:959. 2. Chen AL, et al. 1999. Immunity. 11:689. 3. Croft M. 2010. Annu. Rev. Immunol. 28:57. 4. Withers DR, et al. 2009. J. Immunol. 183:5079. 5. Klinger M, et al. 2009. J. Immunol. 182:4581. |
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| Gene ID | 7293 |
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| UniProt | View information about CD134 on UniProt.org |
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| Clone | Ber-ACT35 (ACT35) |
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| Regulatory Status | RUO |
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| Other Names | ACT35 antigen, tumor necrosis factor receptor superfamily member 4 (TNFRSF4) |
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| Isotype | Mouse IgG1, κ |
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