Biolegend, 350104, Alexa Fluor® 647 anti-human CD294 (CRTH2) Antibody
100tests
Catalog Number: 350104
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Rat cell line TART/B19-12.10 transfected with human CRTH2 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | This product is sold under license agreement with BML, Inc for research use only. |
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| Application References(PubMed link indicates BioLegend citation) | Nagata K, et al. 1999. J. Immunol. 162:1278. (FC) De Fanis U, et al. 2007. Blood 109:4343. (FC) Tsuda H, et al. 2001. Clin. Exp. Immunol. 123:105. (FC) Hamada K, et al. 2004. Allergology International 53:179. (FC) |
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| Product Citations | Mashayekhi M, et al. 2022. Prostaglandins Other Lipid Mediat. 158:106604. PubMed Witkowski M, et al. 2021. Nature. 600:295. PubMed Wright AKA, et al. 2017. J Allergy Clin Immunol. 140:1430. PubMed Vijayakumar B, et al. 2022. Immunity. . PubMed Silver J, et al. 2020. Methods Mol Biol. 2121:115. PubMed Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed |
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| RRID | AB_10640066 (BioLegend Cat. No. 350103) AB_10642025 (BioLegend Cat. No. 350104) |
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| Structure | Seven-transmembrane protein coupled with G proteins |
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| Distribution | Effector T helper 2 (Th2) cells, eosinophils, basophils |
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| Function | Chemotaxis of eosinophils, basophils and Th2 cells, prevention of apoptosis in Th2 cells |
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| Interaction | Heterotrimeric G proteins |
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| Ligand/Receptor | Prostaglandin D2 |
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| Cell Type | Basophils, Eosinophils, Th2 |
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| Biology Area | Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Luster AD and Tager AM. 2004. Nat. Rev. Immunol. 4:711. 2. Monneret G, et al. 2001. Blood 98:1942. 3. Xue L, et al. 2009. J. Immunol. 182:7580. 4. Schratl P, et al. 2007. J. Immunol. 179:4792. |
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| Gene ID | 11251 |
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| UniProt | View information about CD294 on UniProt.org |
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| Clone | BM16 |
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| Regulatory Status | RUO |
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| Other Names | Chemoattractant receptor-homologous molecule expressed on Th2 cells, GPR44, G-protein coupled receptor 44, DP2 |
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| Isotype | Rat IgG2a, κ |
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