Biolegend, 350209, Alexa Fluor® 647 anti-human CD103 (Integrin αE) Antibody
25tests
Catalog Number: 350209
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| Verified Reactivity | Human |
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| Reported Reactivity | Cynomolgus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | HTLV-1 induced human T cell line MAPS16 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: Western Blotting1, immunoprecipitation1, and immunohistochemical staining of frozen tissue sections1. |
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| Application References(PubMed link indicates BioLegend citation) | Kruschwitz M, et al. 1991. J. Clin. Pathol. 44:636. (WB, IP, IHC-F) Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) |
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| Product Citations | Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed |
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| RRID | AB_10640870 (BioLegend Cat. No. 350209) AB_10645641 (BioLegend Cat. No. 350210) |
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| Structure | Type I transmembrane glycoprotein, integrin family; can be cleaved into 150 kD and 25 kD chains; associated with β7 integrin |
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| Distribution | Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells; expressed on hairy cell leukemia |
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| Function | Retention and activation of CD103+ lymphocytes in the intestinal epithelium, regulation of tissue-specific T cell homing |
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| Ligand/Receptor | E-Cadherin |
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| Cell Sources | Integrin β7 |
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| Cell Targets | Integrin β7 |
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| Cell Type | Dendritic cells, Lymphocytes, T cells, Tregs |
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| Biology Area | Cell Biology, Immunology, Neuroscience, Synaptic Biology |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Parker CM, et al. 1992. P. Natl. Acad. Sci. USA 89:1924. 2. Kruschwitz M, et al. 1991. J. Clin. Pathol. 44:636. 3. Schon MP, et al. 1999. J. Immunol. 162:6641. 4. Shaw SK, et al. 1994. J. Biol. Chem. 269:6016. |
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| Gene ID | 3682 |
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| UniProt | View information about CD103 on UniProt.org |
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| Clone | Ber-ACT8 |
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| Regulatory Status | RUO |
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| Workshop | V A067 |
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| Other Names | Integrin alpha E (ITGAE) |
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| Isotype | Mouse IgG1, κ |
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