Biolegend, 350808, APC anti-human CD325 (N-Cadherin) Antibody
100tests
Catalog Number: 350808
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Recombinant human N-cadherin extracellular domain |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. |
| Excitation Laser | Red Laser (633 nm) |
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| Application Notes |
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| The mAb 8C11 recognizes the amino acids 92–593 of CD325, located between the extracellular cadherin structural domain (EC) 3 and 4. Additional reported applications (for the relevant formats) include: immunofluorescence1,3,6, motility inhibition of N-cadherin-expressing cells2, and Western blot2,4. |
| Application References(PubMed link indicates BioLegend citation) | Navarro P, et al. 1998. J. Cell Biol. 140:1475. (IF) Kim JB. 2000. J. Cell Biol. 151:1193. (Block, WB) Puch S, et al. 2001. J. Cell. Sci. 114:1567. (IF) Wahl JK. 3rd, et al. 2003. J. Biol. Chem. 278:17269. (WB) Wein F, et al. 2010. Stem. Cell Res. 4:129. (FC) Jaggi M, et al. 2002. Cell. Commun. Adhes. 9:103. (IF) |
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| Product Citations | Theodoraki MN, et al. 2018. Oncoscience. 5:75. PubMed Tian R, et al. 2019. Neuron. 104:239. PubMed Nakanishi M, et al. 2019. Cell. 177:910. PubMed Norgard RJ, et al. 2021. EMBO Rep. 22:e51872. PubMed |
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| RRID | AB_10983063 (BioLegend Cat. No. 350807) AB_10960145 (BioLegend Cat. No. 350808) |
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| Structure | Member of cadherin family, Single pass transmembrane protein, extracellular region consist of five EC domains, one cytoplasmic domain, 130 kD |
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| Distribution | Stem cells, myeloblasts, endothelial cells, fibroblasts, neural and muscle tissues, some types of carcinoma cells |
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| Function | Embryonic development, maintain tissue architecture, cell sorting and cell adhesion in tissues, promote motility |
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| Interaction | Catenins |
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| Cell Type | Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells |
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| Biology Area | Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Colomiere M, et al. 2009. Brit. J. Cancer 100:134. 2. Yan W, et al. 2010. J. Biol. Chem. 285:14042. 3. Mosnier JF, et al. 2009. Mod. Pathol. 22:182. 4. Gao L, et al. 2010. Stem Cells 28:564. |
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| Gene ID | 1000 |
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| UniProt | View information about CD325 on UniProt.org |
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| Clone | 8C11 |
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| Regulatory Status | RUO |
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| Other Names | Cadherin-2, Neural cadherin |
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| Isotype | Mouse IgG1, κ |
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