Biolegend, 350816, Alexa Fluor® 594 anti-human CD325 (N-Cadherin) Antibody
100microg
Catalog Number: 350816
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Recombinant human N-cadherin extracellular domain |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Application Notes |
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| The mAb 8C11 recognizes the amino acids 92–593 of CD325, located between the extracellular cadherin structural domain (EC) 3 and 4. Additional reported applications (for the relevant formats) include: immunofluorescence1,3,6, motility inhibition of N-cadherin-expressing cells2, and Western blot2,4. |
| Application References(PubMed link indicates BioLegend citation) | Navarro P, et al. 1998. J. Cell Biol. 140:1475. (IF) Kim JB. 2000. J. Cell Biol. 151:1193. (Block, WB) Puch S, et al. 2001. J. Cell. Sci. 114:1567. (IF) Wahl JK. 3rd, et al. 2003. J. Biol. Chem. 278:17269. (WB) Wein F, et al. 2010. Stem. Cell Res. 4:129. (FC) Jaggi M, et al. 2002. Cell. Commun. Adhes. 9:103. (IF) |
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| Product Citations | Aboulkheyr Es H, et al. 2021. J Cell Physiol. 236:3918. PubMed |
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| RRID | AB_2563226 (BioLegend Cat. No. 350816) |
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| Structure | Member of cadherin family, Single pass transmembrane protein, extracellular region consist of five EC domains, one cytoplasmic domain, 130 kD |
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| Distribution | Stem cells, myeloblasts, endothelial cells, fibroblasts, neural and muscle tissues, some types of carcinoma cells |
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| Function | Embryonic development, maintain tissue architecture, cell sorting and cell adhesion in tissues, promote motility |
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| Interaction | Catenins |
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| Cell Type | Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells |
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| Biology Area | Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Colomiere M, et al. 2009. Brit. J. Cancer 100:134. 2. Yan W, et al. 2010. J. Biol. Chem. 285:14042. 3. Mosnier JF, et al. 2009. Mod. Pathol. 22:182. 4. Gao L, et al. 2010. Stem Cells 28:564. |
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| Gene ID | 1000 |
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| UniProt | View information about CD325 on UniProt.org |
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| Clone | 8C11 |
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| Regulatory Status | RUO |
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| Other Names | Cadherin-2, Neural cadherin |
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| Isotype | Mouse IgG1, κ |
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