Biolegend, 351324, PE/Cyanine5 anti-human CD127 (IL-7Rα) Antibody
100tests
Catalog Number: 351324
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| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Baboon, Cynomolgus, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Recombinant human CD127 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with PE/Cyanine5 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. |
| Excitation Laser | Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm) |
Answer:PE/Cyanine5 Anti-Human CD127 (IL-7Rα) Antibody, 351324.
Answer:Human CD127; PE/Cyanine5; Flow cytometry.
Answer:PE/Cyanine5 anti-human CD127 is a fluorescent antibody used to identify and characterize human cells expressing the interleukin-7 receptor alpha chain.
PE/Cyanine5 anti-human CD127 is a fluorescent antibody used to identify and characterize human cells expressing the interleukin-7 receptor alpha chain. CD127 is commonly assessed in lymphocyte phenotyping and is informative for subsets of T cells, including regulatory-T-cell workflows when interpreted together with other markers. The PE/Cyanine5 tandem fluorophore enables multicolor flow cytometry with an appropriately configured laser and detector set. This reagent supports comparative analysis of CD127 expression in mixed immune-cell samples and panel designs involving additional surface markers. Fluorescence spillover and staining conditions should be validated for the instrument and sample type. The specific vial size and clone are not assumed from the antibody family.
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For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Application Notes | Additional reported (for the relevant formats) application: proteogenomics1. |
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| Application References(PubMed link indicates BioLegend citation) | Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG) |
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| Product Citations | Adamo S, et al. 2023. Cell Host Microbe. 31:928. PubMed Alam A, et al. 2022. Cancer Cell. 40:153. PubMed Pandit H, et al. 2023. iScience. 26:105929. PubMed Zhang B, et al. 2022. J Exp Med. 219:. PubMed Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed Gu C, et al. 2021. Front Immunol. 12:678036. PubMed |
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| RRID | AB_10917390 (BioLegend Cat. No. 351323) AB_10915554 (BioLegend Cat. No. 351324) |
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| Structure | Type I transmembrane glycoprotein, associates with CD132, 60-90 kD |
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| Distribution | Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells |
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| Function | T cell and immature B cell proliferation and development |
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| Ligand/Receptor | IL-7 |
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| Cell Type | B cells, T cells, Thymocytes, Tregs |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors |
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| Antigen References | 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. 2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503. 3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610. 4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403. 5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707. 6. Liu W, et al. 2006. J. Exp. Med. 203:1701. |
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| Gene ID | 3575 |
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| UniProt | View information about CD127 on UniProt.org |
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| Clone | A019D5 |
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| Regulatory Status | RUO |
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| Other Names | IL-7 receptor α chain, IL-7Rα |
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| Isotype | Mouse IgG1, κ |
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