Biolegend, 352314, Alexa Fluor® 647 anti-human CD138 (Syndecan-1) Antibody
100tests
Catalog Number: 352314
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| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Baboon, Cynomolgus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human SDC1 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | The epitope recognized by MI15 is found within the ectodomain of the CD138 core protein. It has been reported that MI15 blocks the binding of clone B-B4 but not clone DL-101 by flow cytometric analysis. Clones DL-101 and MI15 exhibit differential staining patterns on in vitro generated plasma cells and some CD138+ cell lines.2 Additional reported applications (for the relevant formats of this clone) include: immunohistochemical staining in paraffin blocks of tissue sections1. |
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| Application References(PubMed link indicates BioLegend citation) | Osama MA. 2010. Int. J. Clin. Exp. Pathol. 3:280. (IHC) Itoua MR, et al. 2014. Biomed. Res. Int. 2014:536482. PubMed |
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| RRID | AB_2687335 (BioLegend Cat. No. 352313) AB_2687336 (BioLegend Cat. No. 352314) |
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| Structure | 100-200 kD type I integral transmembrane glycoprotein |
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| Distribution | Plasma cells, pre-B cells, epithelial cells, endothelial cells |
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| Function | Adhesion, control cell morphology, regulate cell growth |
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| Ligand/Receptor | FGFb, collagen, fibronectin |
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| Cell Type | B cells, Endothelial cells, Epithelial cells, Plasma cells |
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| Biology Area | Cell Adhesion, Cell Biology, Immunology |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Sanderson RD, et al. 1992. Cell. Regul. 1:27. 2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication. |
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| Gene ID | 6382 |
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| UniProt | View information about CD138 on UniProt.org |
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| Clone | DL-101 |
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| Regulatory Status | RUO |
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| Workshop | V B045 |
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| Other Names | Syndecan-1, B-B4 |
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| Isotype | Mouse IgG1, κ |
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