Biolegend, 352908, Alexa Fluor® 488 anti-human EGFR Antibody
100tests
Catalog Number: 352908
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Non-small cell lung cancer (NSCLC) cell line NCI-H322 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested ICC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Blue Laser (488 nm) |
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| Application References(PubMed link indicates BioLegend citation) | Yamaguchi M, et al. 2009. The 15th Annual Meeting Japan Society of Gene Therapy. p1056. Abstract 92. |
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| Product Citations | Arshad N, et al. 2018. J Biol Chem. 293:9555. PubMed Pham TC, et al. 2021. J Extracell Vesicles. e12057:10. PubMed Kitamura Y, et al. 2021. Sci Adv. 7: . PubMed Mahameed M, et al. 2020. Nat Commun. 11:1304. PubMed Jayasinghe MK, et al. 2022. Theranostics. 12:3288. PubMed Subham S, et al. 2022. Breast Cancer Res Treat. Online ahead of print. PubMed Marrocco I, et al. 2021. EMBO Mol Med. 13:e13144. PubMed Mahameed M, et al. 2019. Cell Death Dis. 10:300. PubMed Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed Staudte S, et al. 2022. Cancers (Basel). 14:. PubMed Xuan Z, et al. 2020. Am J Cancer Res. 0.458333333. PubMed Liu H, et al. 2020. Cancer Discov. 1.268055556. PubMed Kahn S, et al. 2016. EMBO Mol Med. 8: 511 - 526. PubMed Leonard B, et al. 2018. Cancer Res. 78:4331. PubMed |
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| RRID | AB_11124324 (BioLegend Cat. No. 352907) AB_11126165 (BioLegend Cat. No. 352908) |
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| Structure | Member of the protein kinase superfamily; transmembrane glycoprotein; ligand binding induces dimerization and autophosphorylation |
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| Distribution | Epithelial cells and endothelial cells; frequently found in epithelial carcinomas |
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| Function | Controls cell growth and differentiation |
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| Interaction | MAPK, Akt, JNK |
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| Ligand/Receptor | Members of the epidermal growth factor (EGF) family such as EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor |
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| Cell Type | Endothelial cells, Epithelial cells |
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| Biology Area | Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Synaptic Biology |
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| Molecular Family | CD Molecules, Growth Factors |
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| Antigen References | 1. da Cunha Santos G, et al. 2011. Annu. Rev. Pathol. 6:49. 2. Gusterson BA and Hunter KD. 2009. Lancet Oncol. 10:522. 3. Mano M and Humblet Y. 2008. Nat. Clin. Pract. Oncol. 5:415. 4. Pao W and Chmielecki J. 2010. Nat. Rev. Cancer 10:760. |
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| Gene ID | 1956 |
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| UniProt | View information about EGFR on UniProt.org |
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| Clone | AY13 |
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| Regulatory Status | RUO |
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| Other Names | Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, HER1 |
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| Isotype | Mouse IgG1, κ |
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