Biolegend, 352917, Alexa Fluor® 647 anti-human EGFR Antibody
25tests
Catalog Number: 352917
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Non-small cell lung cancer (NSCLC) cell line NCI-H322 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
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| Excitation Laser | Red Laser (633 nm) |
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| Application References(PubMed link indicates BioLegend citation) | Yamaguchi M, et al. 2009. The 15th Annual Meeting Japan Society of Gene Therapy. p1056. Abstract 92. |
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| Product Citations | Slamecka J, et al. 2024. iScience. 110874:27. PubMed |
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| RRID | AB_2650983 (BioLegend Cat. No. 352917) AB_2650984 (BioLegend Cat. No. 352918) |
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| Structure | Member of the protein kinase superfamily; transmembrane glycoprotein; ligand binding induces dimerization and autophosphorylation |
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| Distribution | Epithelial cells and endothelial cells; frequently found in epithelial carcinomas |
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| Function | Controls cell growth and differentiation |
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| Interaction | MAPK, Akt, JNK |
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| Ligand/Receptor | Members of the epidermal growth factor (EGF) family such as EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor |
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| Cell Type | Endothelial cells, Epithelial cells |
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| Biology Area | Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Synaptic Biology |
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| Molecular Family | CD Molecules, Growth Factors |
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| Antigen References | 1. da Cunha Santos G, et al. 2011. Annu. Rev. Pathol. 6:49. 2. Gusterson BA and Hunter KD. 2009. Lancet Oncol. 10:522. 3. Mano M and Humblet Y. 2008. Nat. Clin. Pract. Oncol. 5:415. 4. Pao W and Chmielecki J. 2010. Nat. Rev. Cancer 10:760. |
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| Gene ID | 1956 |
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| UniProt | View information about EGFR on UniProt.org |
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| Clone | AY13 |
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| Regulatory Status | RUO |
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| Other Names | Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, HER1 |
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| Isotype | Mouse IgG1, κ |
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