Biolegend, 353320, Alexa Fluor® 488 anti-human Perforin Antibody
100tests
Catalog Number: 353320
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Blue Laser (488 nm) |
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| Product Citations | Ma X, et al. 2021. Cell Metabolism. 33(5):1001-1012.e5. PubMed |
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| RRID | AB_2783254 (BioLegend Cat. No. 353319) AB_2783255 (BioLegend Cat. No. 353320) |
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| Structure | 70 kD |
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| Distribution | CTL, NK (cytoplasmic granules) |
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| Function | Mediates targeted cell lysis. |
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| Cell Type | NK cells, Tregs |
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| Biology Area | Cell Biology, Immunology, Innate Immunity, Neuroscience |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361. 2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735. |
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| Gene ID | 5551 |
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| UniProt | View information about Perforin on UniProt.org |
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| Clone | B-D48 |
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| Regulatory Status | RUO |
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| Other Names | PRF1, P1, PFP, HPLH2 |
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| Isotype | Mouse IgG1 |
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